鸡下丘脑血管活性肠肽受体的存在

Presence of vasoactive intestinal peptide receptor in the hen hypothalamus.

作者信息

Gonzales S M, Kawashima M, Kamiyoshi M, Tanaka K, Ichinoe K

机构信息

Department of Zootechnical Science, Tokyo University of Agriculture, Japan.

出版信息

Endocr J. 1995 Apr;42(2):179-86. doi: 10.1507/endocrj.42.179.

DOI:10.1507/endocrj.42.179

PMID:7627262

Abstract

Radioligand assays of the membrane fraction of hen hypothalamic tissues involving the preoptic (HPOA) or median eminence (HMEA) areas revealed the presence of a specific binding component to chicken vasoactive intestinal peptide (cVIP) having properties of a receptor. The equilibrium dissociation constant (Kd) was 0.70 +/- 0.07 nM (Mean +/- SEM; N = 5) in HPOA and 1.02 +/- 0.15 nM (N = 5) in HMEA as estimated by Scatchard analysis of saturation studies, and was 0.91 +/- 0.11 nM (N = 3) (HPOA) and 1.25 +/- 0.09 nM (N = 3) (HMEA) as determined by a kinetic analysis. The maximum binding capacity (Bmax) obtained by Scatchard analysis was 167 +/- 19 fmol/mg protein (N = 5) (HPOA) and 133 +/- 17 fmol/mg protein (N = 5) (HMEA). The Kd and Bmax values obtained by Scatchard analysis were similar in the two areas of the hypothalamus and in both laying and nonlaying hens. Administration of cVIP in vivo caused a decrease in specific cVIP binding. These results suggest the presence of a VIP receptor in the hen hypothalamus.

摘要

对母鸡下丘脑组织视前区（HPOA）或正中隆起区（HMEA）的膜部分进行放射性配体分析，结果显示存在一种对鸡血管活性肠肽（cVIP）具有受体特性的特异性结合成分。通过饱和研究的Scatchard分析估计，HPOA中的平衡解离常数（Kd）为0.70±0.07 nM（平均值±标准误；N = 5），HMEA中的为1.02±0.15 nM（N = 5）；通过动力学分析测定，HPOA中的为0.91±0.11 nM（N = 3），HMEA中的为1.25±0.09 nM（N = 3）。通过Scatchard分析获得的最大结合容量（Bmax）为167±19 fmol/mg蛋白质（N = 5）（HPOA）和133±17 fmol/mg蛋白质（N = 5）（HMEA）。通过Scatchard分析获得的Kd和Bmax值在下丘脑的这两个区域以及产蛋和非产蛋母鸡中相似。体内注射cVIP导致特异性cVIP结合减少。这些结果表明母鸡下丘脑中存在VIP受体。