Takahashi K, Shichijo S, Noguchi M, Hirohata M, Itoh K
Department of Immunology, Kurume University School of Medicine, Japan.
Cancer Res. 1995 Aug 15;55(16):3478-82.
The MAGE genes encoding tumor rejection antigens recognized by CTLs are expressed at the mRNA level in various cancers and in the testis but not in the other normal tissues. The expression of MAGE-1 or MAGE-4 protein in the testicular cells was studied immunohistochemically with the antibodies to the recombinant MAGE-1 or MAGE-4 protein. Both proteins were identified in the nucleus and cytoplasm of spermatogonia and in primary spermatocytes but not in spermatids or Sertoli's cells. Therefore, MAGE proteins are normal tissue antigens compartmentalized in particular testicular cells playing an important role in the early phase of the spermatogenesis.
编码被细胞毒性T淋巴细胞（CTLs）识别的肿瘤排斥抗原的MAGE基因，在多种癌症和睾丸组织中以mRNA水平表达，但在其他正常组织中不表达。用针对重组MAGE-1或MAGE-4蛋白的抗体，通过免疫组织化学方法研究了睾丸细胞中MAGE-1或MAGE-4蛋白的表达情况。在精原细胞和初级精母细胞的细胞核和细胞质中均鉴定出这两种蛋白，而在精子细胞或支持细胞中未鉴定出。因此，MAGE蛋白是正常组织抗原，局限于特定的睾丸细胞中，在精子发生的早期阶段发挥重要作用。
