Iozzo R V, Eichstetter I, Danielson K G
Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Cancer Res. 1995 Aug 15;55(16):3495-9.
The Wnt-5A gene codes for a secreted cysteine-rich growth factor that mediates cell to cell signaling via a paracrine mechanism during development and ontogeny. We have recently determine the genomic organization and chromosomal mapping of the human Wnt-5A, and observed distinct patterns of expression in developing human embryos. In this report, we have performed a detailed expression analysis of 100 adult human tissues and tumors and 10 human cell lines. Our data show a widespread expression of Wnt-5A in adult tissues and cells, and aberrant mRNA levels in lungs, breast, and prostate carcinomas and in melanomas. The up-regulation of Wnt-5A in human malignancy was not due to either gene rearrangement or amplification. These findings document an abnormal expression of this growth factor in malignancy and implicate Wnt-5A in the genesis of human cancer.
Wnt-5A基因编码一种分泌型富含半胱氨酸的生长因子，该因子在发育和个体发生过程中通过旁分泌机制介导细胞间信号传导。我们最近确定了人类Wnt-5A的基因组结构和染色体定位，并观察到其在人类胚胎发育过程中独特的表达模式。在本报告中，我们对100种成人人类组织和肿瘤以及10种人类细胞系进行了详细的表达分析。我们的数据显示Wnt-5A在成人组织和细胞中广泛表达，在肺癌、乳腺癌、前列腺癌和黑色素瘤中mRNA水平异常。人类恶性肿瘤中Wnt-5A的上调并非由于基因重排或扩增。这些发现证明了这种生长因子在恶性肿瘤中的异常表达，并提示Wnt-5A与人类癌症的发生有关。
