Padh H, Tanjore S
Center for Biotechnology, Northwestern University, Evanston, IL 60208, USA.
FEBS Lett. 1995 Jul 17;368(2):358-62. doi: 10.1016/0014-5793(95)00656-t.
Earlier studies have shown that in Dictyostelium discoideum, a buoyant membrane fraction contained approximately 90% of the vacuolar proton pump (V-H(+)-ATPase) activity, leading to its designation acidosomes. It was proposed that acidosomes may be involved in endocytosis, specially in the acidification of endosomes. In this study we further investigated the putative function(s) of acidosomes. The findings suggest that acidosomes contain abundant receptors for cyclic AMP (CAR1) and that it may be the site for recycling of internalized receptors. Acidosomes also contain an abundance of Rab4 (Bush et al. 1994), a marker for early endosomes. By these criteria, we suggest that the acidosomes are analogous to early or recycling endosome present in mammalian cells. These findings suggest that the structure earlier defined biochemically, morphologically and immunologically as acidosomes may represent early and/or recycling endosomes in this protist.
早期研究表明，在盘基网柄菌中，一个有浮力的膜组分含有约90%的液泡质子泵（V-H(+)-ATP酶）活性，因此将其命名为酸泡体。有人提出，酸泡体可能参与胞吞作用，特别是在内体的酸化过程中。在本研究中，我们进一步探究了酸泡体的假定功能。研究结果表明，酸泡体含有丰富的环磷酸腺苷受体（CAR1），并且它可能是内化受体循环利用的场所。酸泡体还含有大量的Rab4（布什等人，1994年），这是早期内体的一个标志物。基于这些标准，我们认为酸泡体类似于哺乳动物细胞中存在的早期或循环内体。这些发现表明，先前在生物化学、形态学和免疫学上定义为酸泡体的结构可能代表了这种原生生物中的早期和/或循环内体。
