Vos J P, Giudici M L, van der Bijl P, Magni P, Marchesini S, van Golde L M, Lopes-Cardozo M
Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.
FEBS Lett. 1995 Jul 17;368(2):393-6. doi: 10.1016/0014-5793(95)00695-6.
In most cell types sphingomyelin is synthesized predominantly in the cis-medial compartments of the Golgi stacks whereas the contribution of the plasma membrane is much lower. The aim of this study was to assess the contribution of both compartments to the synthesis of sphingomyelin in myelinating cells. Therefore, oligodendrocytes from rat spinal cord were incubated in culture with fluorescently- or radiolabelled ceramides, and the effects of a block in the vesicular flow (monensin, brefeldin A, low temperature) on surface synthesis of sphingomyelin were evaluated. The results indicate that approximately 50% of the sphingomyelin synthase is present at the plasma and myelin membranes of oligodendrocytes.
在大多数细胞类型中，鞘磷脂主要在高尔基体堆叠的顺式-中间区室中合成，而质膜的贡献则低得多。本研究的目的是评估这两个区室对髓鞘形成细胞中鞘磷脂合成的贡献。因此，将来自大鼠脊髓的少突胶质细胞与荧光或放射性标记的神经酰胺一起在培养物中孵育，并评估囊泡运输受阻（莫能菌素、布雷菲德菌素A、低温）对鞘磷脂表面合成的影响。结果表明，约50%的鞘磷脂合酶存在于少突胶质细胞的质膜和髓鞘膜上。
