Cheville J C, Greiner T, Robinson R A, Benda J A
Department of Pathology, University of Iowa College of Medicine, USA.
Hum Pathol. 1995 Jul;26(7):753-7. doi: 10.1016/0046-8177(95)90223-6.
Placentas with hydropic change may be hydropic degeneration (HD) or gestational trophoblastic disease (GTD), partial (PM) or complete (CM) hydatidiform mole. The separation of HD from PM and PM from CM by histological findings may be problematic in some cases and can be clarified with ploidy analysis. Fluorescence in situ hybridization (FISH) using a probe to chromosome 7 (D7Z1) was applied to tissue cut from paraffin blocks from 10 histologically representative cases each of HD, PM, and CM on which ploidy had been previously confirmed by flow cytometry from paraffin embedded tissue. Villous stromal cells and nonproliferative trophoblast were examined for number of signals/cell and percentage of cells/placenta with three hybridization signals. The mean number of hybridization signals/cell was HD 1.14; PM 1.79; and CM 1.17, with statistical significance between HD and PM (P < .0001), and PM and CM (P < .0001). The mean percentage of cells/placenta with three hybridization signals was HD 1.10%, PM 23.1%, and CM 2.11%, with statistical significance between HD and PM (P < .0001), and PM and CM (P < .0001). In addition, there was no overlap in the mean percentage of cells with three hybridization signals between HD and PM, and PM and CM. Chromosome 2 probe (D2Z1) was applied to tissues that had three chromosome 7 signals to exclude trisomy, and in all cases three signals were present confirming triploidy in PM. FISH can identify diploid and triploid hydropic placentas in paraffin-embedded tissue to assist in differentiating HD from PM, and PM from CM.
出现水肿性改变的胎盘可能是水肿变性（HD）或妊娠滋养细胞疾病（GTD），部分性（PM）或完全性（CM）葡萄胎。在某些情况下，通过组织学结果区分HD与PM以及PM与CM可能存在问题，而倍性分析可以阐明这一点。使用针对7号染色体（D7Z1）的探针进行荧光原位杂交（FISH），应用于从石蜡块切取的组织，这些组织来自HD、PM和CM各10例具有组织学代表性的病例，此前已通过石蜡包埋组织的流式细胞术确认了倍性。检查绒毛间质细胞和非增殖性滋养细胞的信号数/细胞以及具有三个杂交信号的细胞/胎盘百分比。杂交信号/细胞的平均数量为HD 1.14；PM 1.79；CM 1.17，HD与PM之间（P <.0001）以及PM与CM之间（P <.0001）具有统计学意义。具有三个杂交信号的细胞/胎盘平均百分比为HD 1.10%，PM 23.1%，CM 2.11%，HD与PM之间（P <.0001）以及PM与CM之间（P <.0001）具有统计学意义。此外，HD与PM以及PM与CM之间具有三个杂交信号的细胞平均百分比没有重叠。将2号染色体探针（D2Z1）应用于具有三个7号染色体信号的组织以排除三体性，在所有病例中均出现三个信号，证实PM为三倍体。FISH可以识别石蜡包埋组织中的二倍体和三倍体水肿性胎盘，以协助区分HD与PM以及PM与CM。
