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推测的嗜热栖热菌TATA结合蛋白序列的翻译产物是一种在结构和功能上与甲烷球菌转录因子B相关的转录因子。

The translation product of the presumptive Thermococcus celer TATA-binding protein sequence is a transcription factor related in structure and function to Methanococcus transcription factor B.

作者信息

Hausner W, Thomm M

机构信息

Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität zu Kiel, Federal Republic of Germany.

出版信息

J Biol Chem. 1995 Jul 28;270(30):17649-51. doi: 10.1074/jbc.270.30.17649.

DOI:10.1074/jbc.270.30.17649
PMID:7629058
Abstract

A gene for a putative homolog of TATA-binding protein (TBP) from Thermococcus celer has been expressed in Escherichia coli, and the function of the purified recombinant protein was studied in a Methanococcus-derived cell-free transcription system. Thermococcus TBP can replace archaeal transcription factor B (aTFB) in cell-free transcription reactions. This transcriptional activation is TATA box-dependent and occurs both on tRNA(Val) and protein-encoding genes as templates indicating that Thermococcus TBP is a general transcription factor. Antibodies raised against Thermococcus TBP bind to Methanococcus aTFB and inhibit a TFB activity. These findings demonstrate that Thermococcus TBP (like eucaryal TBPs) can direct specific transcription from TATA boxes.

摘要

嗜热栖热菌（Thermococcus celer）中一个假定的TATA结合蛋白（TBP）同源基因已在大肠杆菌中表达，并在源自甲烷球菌（Methanococcus）的无细胞转录系统中研究了纯化的重组蛋白的功能。嗜热栖热菌TBP可在无细胞转录反应中替代古菌转录因子B（aTFB）。这种转录激活是TATA框依赖性的，并且以tRNA（Val）和蛋白质编码基因作为模板发生，表明嗜热栖热菌TBP是一种通用转录因子。针对嗜热栖热菌TBP产生的抗体与甲烷球菌aTFB结合并抑制TFB活性。这些发现表明嗜热栖热菌TBP（如真核生物TBP）可以指导从TATA框进行特异性转录。

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Mol Microbiol. 2009 Feb;71(4):989-1002. doi: 10.1111/j.1365-2958.2008.06577.x. Epub 2008 Dec 18.
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A Pyrococcus homolog of the leucine-responsive regulatory protein, LrpA, inhibits transcription by abrogating RNA polymerase recruitment.
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