Galasko G T, Bao Y, Broomfield S J, Hooper N M, Turner A J, Larner J
Department of Pharmacology, University of Virginia School of Medicine, Charlottesville 22908, USA.
J Clin Endocrinol Metab. 1995 Aug;80(8):2419-29. doi: 10.1210/jcem.80.8.7629237.
A novel low mol wt inositol phosphoglycan inhibitor (M tau 1200-1500) of insulin action in rat adipocytes has been partially purified from normal human plasma. This inhibitor, termed fraction V after the first purification step and fraction V3 after the second, is different from other reported serum insulin antagonists. It contains myoinositol, galactosamine, and mannose in approximate molar ratios of 1:1:3.3. The myoinositol has a 1,2-cyclic phosphate substituent, which is essential for the inhibitory activity. Its inhibitory activity is significantly elevated (161%, P < 0.05 for fraction V; 278%, P < 0.05 for fraction V3) in plasma of humans with noninsulin-dependent diabetes mellitus as compared with plasma of nondiabetic controls. These findings represent the first report of a naturally occurring mammalian inositol 1,2-cyclic phosphate containing phosphoglycan related to insulin action.
一种新型的低分子量肌醇磷酸聚糖胰岛素作用抑制剂（M tau 1200 - 1500）已从正常人血浆中部分纯化出来，该抑制剂作用于大鼠脂肪细胞。这种抑制剂在第一步纯化后称为组分V，第二步纯化后称为组分V3，它不同于其他已报道的血清胰岛素拮抗剂。它含有肌醇、半乳糖胺和甘露糖，摩尔比约为1:1:3.3。肌醇具有1,2 - 环磷酸取代基，这对抑制活性至关重要。与非糖尿病对照者的血浆相比，非胰岛素依赖型糖尿病患者血浆中的这种抑制剂的抑制活性显著升高（组分V升高161%，P < 0.05；组分V3升高278%，P < 0.05）。这些发现代表了首次报道与胰岛素作用相关的天然存在的含1,2 - 环磷酸肌醇的哺乳动物磷酸聚糖。
