Kim M H, Oka Y, Amano M, Kobayashi M, Okuzawa K, Hasegawa Y, Kawashima S, Suzuki Y, Aida K
Department of Fisheries, Faculty of Agriculture, University of Tokyo, Japan.
J Comp Neurol. 1995 May 22;356(1):72-82. doi: 10.1002/cne.903560105.
The immunocytochemical distribution of salmon gonadotropin-releasing hormone (sGnRH) and chicken GnRH-II (cGnRH-II) neurons in the brain of goldfish was examined using respective antisera. Salmon GnRH-immunoreactive (ir) cell bodies were localized in the area between the olfactory nerve and the olfactory bulb (the terminal nerve ganglion), the ventral telencephalon, the preoptic area, and the hypothalamus. Chicken GnRH-II-ir cell bodies were observed in the same areas as were those of sGnRH, although the number of cell bodies were fewer than those of sGnRH. In addition, chicken GnRH-II-ir cell bodies were also observed in the midbrain tegmentum where no sGnRH-ir cell bodies were found. Both sGnRH-ir and cGnRH-II-ir fibers were distributed not only in the hypothalamus and the pituitary gland but also in various brain areas from the olfactory bulb to the spinal cord. The wide distribution of GnRH-ir fibers suggests that in the goldfish, sGnRH and cGnRH-II not only regulate gonadotropin release from the pituitary gland but also function as neuromodulators in various brain regions.
利用相应的抗血清检测了金鱼脑中鲑鱼促性腺激素释放激素（sGnRH）和鸡促性腺激素释放激素II（cGnRH-II）神经元的免疫细胞化学分布。鲑鱼GnRH免疫反应性（ir）细胞体定位于嗅神经与嗅球之间的区域（终末神经节）、腹侧端脑、视前区和下丘脑。鸡GnRH-II-ir细胞体在与sGnRH相同的区域被观察到，尽管细胞体的数量比sGnRH的少。此外，在中脑被盖中也观察到鸡GnRH-II-ir细胞体，而在该区域未发现sGnRH-ir细胞体。sGnRH-ir和cGnRH-II-ir纤维不仅分布在下丘脑和垂体，还分布在从嗅球到脊髓的各个脑区。GnRH-ir纤维的广泛分布表明，在金鱼中，sGnRH和cGnRH-II不仅调节垂体促性腺激素的释放，还在各个脑区作为神经调质发挥作用。
