Kawanishi J, Kato J, Sasaki K, Fujii S, Watanabe N, Niitsu Y
Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine.
Nihon Rinsho. 1995 Jul;53(7):1590-4.
E-cadherin is a calcium dependent cell-cell adhesion molecule. In cancer tissue, detachment of the adhesion system is indispensable for invasion and metastasis of cancer cells. We have investigated mechanism of the dysfunction of E-cadherin-dependent cell-cell adhesion system in gastric carcinoma cells. Although the high expression of E-cadherin in a scirrhous gastric cancer cell line HSC-39, the function of E-cadherin was completely abolished. Western blotting of beta-catenin in HSC-39 cells demonstrated that a truncated beta-catenin was detected. The truncation was due to partial deletion of beta-catenin DNA. It was concluded that in HSC-39 loss of E-cadherin dependent cell-cell adhesion was due to mutation of beta-catenin gene.
E-钙黏蛋白是一种钙依赖性细胞间黏附分子。在癌组织中，黏附系统的解离对于癌细胞的侵袭和转移是不可或缺的。我们研究了胃癌细胞中E-钙黏蛋白依赖性细胞间黏附系统功能障碍的机制。尽管在硬癌胃癌细胞系HSC-39中E-钙黏蛋白高表达，但其功能却完全丧失。对HSC-39细胞中β-连环蛋白进行蛋白质印迹分析显示，检测到一种截短的β-连环蛋白。这种截短是由于β-连环蛋白DNA的部分缺失所致。得出的结论是，在HSC-39细胞中，E-钙黏蛋白依赖性细胞间黏附的丧失是由于β-连环蛋白基因的突变。
