Yang H C, McElroy R J, Kreider J W, Marshall R L, Martinie J B, Diamond J
Department of Surgery, Milton S. Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
J Surg Res. 1995 Jul;59(1):205-10. doi: 10.1006/jsre.1995.1155.
We have shown that Fischer 344-->Lewis renal allografts (ALLO) develop chronic rejection which is not detected in Lewis-->Lewis isografts (ISO). The progression of chronic rejection in ALLO can be reversed by retransplantation (RE-TX) of kidneys from ALLO back into syngeneic Fischer 344 recipients. The purpose of this study was to assess the in situ expression of PDGF-beta, a cytokine associated with wound injury, in ISO, ALLO, and RE-TX. In situ PDGF-beta mRNA expression in kidney sections was assessed early (8 weeks) and late (16 weeks) during the development of chronic rejection. Kidneys from ALLO were transplanted back into syngeneic Fischer recipients at 12 weeks and evaluated for PDGF-beta expression 12 weeks later. Differences in glomerular staining were graded quantitatively on a minimum of 25 glomeruli per section with grade 0, no positive cells in the glomerulus; grade 1, 1 or 2 positive cells; grade 2, 3 or more positive cells in a segmental distribution; and grade 3, > 4 positive cells of moderate intensity in a diffuse distribution. According to this grading system, glomerular PDGF-beta mRNA expression in isografts (N = 6) at 8 and 16 weeks after transplantation was 0.09 +/- 0.03 and 0.2 +/- 0.04, respectively. In allografts (N = 6), PDGF-beta mRNA was significantly higher (P < .001) for the same time periods, 1.28 +/- 0.6 and 1.89 +/- 0.08, respectively. In situ expression of PDGF in retransplants (N = 6) at 24 weeks, 0.07 +/- 0.02, was significantly diminished (P < .001) at 24 weeks compared to allografts at 8 or 16 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)
我们已经表明，费希尔344大鼠到刘易斯大鼠的肾移植（ALLO）会发生慢性排斥反应，而刘易斯大鼠到刘易斯大鼠的同基因移植（ISO）则未检测到这种情况。将肾移植（ALLO）的肾脏再次移植（RE-TX）回同基因的费希尔344受体中，可以逆转慢性排斥反应的进展。本研究的目的是评估血小板衍生生长因子-β（PDGF-β）在同基因移植（ISO）、肾移植（ALLO）和再次移植（RE-TX）中的原位表达，PDGF-β是一种与伤口损伤相关的细胞因子。在慢性排斥反应发展的早期（8周）和晚期（16周），评估肾切片中PDGF-β mRNA的原位表达。在12周时，将肾移植（ALLO）的肾脏再次移植回同基因的费希尔受体中，并在12周后评估PDGF-β的表达。对每切片至少25个肾小球的肾小球染色差异进行定量分级，0级为肾小球中无阳性细胞；1级为1或2个阳性细胞；2级为节段分布中有3个或更多阳性细胞；3级为弥漫分布中有>4个中等强度的阳性细胞。根据该分级系统，移植后8周和16周同基因移植（N = 6）中肾小球PDGF-β mRNA表达分别为0.09±0.03和0.2±0.04。在相同时间段内，肾移植（N = 6）中PDGF-β mRNA显著更高（P <.001），分别为1.28±0.6和1.89±0.08。再次移植（N = 6）在24周时PDGF的原位表达为0.07±0.02，与肾移植在8周或16周时相比，在24周时显著降低（P <.001）。（摘要截断于250字）
