端粒酶RNA基因突变导致的端粒失控性延长

Runaway telomere elongation caused by telomerase RNA gene mutations.

作者信息

McEachern M J, Blackburn E H

机构信息

Department of Microbiology and Immunology, University of California at San Francisco 94143, USA.

出版信息

Nature. 1995 Aug 3;376(6539):403-9. doi: 10.1038/376403a0.

DOI:10.1038/376403a0

PMID:7630414

Abstract

The ribonucleoprotein enzyme telomerase adds telomeric DNA onto chromosome ends and is normally regulated so that telomeric DNA lengths are kept within defined bounds. In the telomerase RNA gene from the yeast Kluyveromyces lactis, specific mutations that alter telomeric DNA sequences result in telomeres elongating to up to 100 times their normal length and impair cell growth. Some mutations cause immediate elongation whereas others behave like genetic time bombs, causing elongation only after a latent period of hundreds of generations.

摘要

核糖核蛋白酶端粒酶将端粒DNA添加到染色体末端，并且通常受到调控，以使端粒DNA长度保持在限定范围内。在乳酸克鲁维酵母的端粒酶RNA基因中，改变端粒DNA序列的特定突变会导致端粒延长至正常长度的100倍，并损害细胞生长。一些突变会导致立即延长，而另一些则表现得像基因定时炸弹，仅在数百代的潜伏期后才导致延长。