Vossen R C, van Dam-Mieras M C, Hornstra G, Zwaal R F
Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), University of Limburg, The Netherlands.
Prostaglandins Leukot Essent Fatty Acids. 1995 May;52(5):341-7. doi: 10.1016/0952-3278(95)90036-5.
In order to study the relationship between the fatty acid (FA) composition of human umbilical vein endothelial cells (HUVEC) and their susceptibility to oxidative stress, we modified their FA composition by long-term culturing in media supplemented with a saturated, monounsaturated, or polyunsaturated FA. Sensitivity of the cellular phospholipids to peroxidation was monitored by measuring conjugated diene formation and decrease of polyunsaturated FAs induced by CuSO4 and H2O2 in liposomes prepared from the respective phospholipid extracts. The extent of phospholipid peroxidation was found to increase with increasing content of polyunsaturated FAs. In addition, the sensitivity of individual polyunsaturated FAs to peroxidation was directly proportional to the number of double bonds present. However, no unequivocal relationship was observed between conjugated diene formation and the phospholipid unsaturation index (an indicator for the combined effect of number of double bonds and polyunsaturated FA contents of the membrane phospholipids). The results suggest that long-term FA modification of endothelial cells differentially alters the sensitivity of their membrane phospholipids to peroxidation: long-term modification with oleic acid may protect against lipid peroxidation, whereas linoleic acid may increase sensitivity to peroxidation. In contrast to what might be expected, long-term modification of endothelial cells with eicosapentaenoic or docosahexaenoic acid does not increase the sensitivity of phospholipids to peroxidation.
为了研究人脐静脉内皮细胞（HUVEC）的脂肪酸（FA）组成与其对氧化应激的敏感性之间的关系，我们通过在补充了饱和脂肪酸、单不饱和脂肪酸或多不饱和脂肪酸的培养基中进行长期培养来改变其FA组成。通过测量共轭二烯的形成以及由硫酸铜和过氧化氢在由各自磷脂提取物制备的脂质体中诱导的多不饱和脂肪酸的减少，来监测细胞磷脂对过氧化的敏感性。发现磷脂过氧化的程度随着多不饱和脂肪酸含量的增加而增加。此外，单个多不饱和脂肪酸对过氧化的敏感性与双键的数量成正比。然而，在共轭二烯的形成与磷脂不饱和度指数（膜磷脂双键数量和多不饱和脂肪酸含量综合效应的指标）之间未观察到明确的关系。结果表明，内皮细胞的长期FA修饰会不同程度地改变其膜磷脂对过氧化的敏感性：用油酸进行长期修饰可能防止脂质过氧化，而亚油酸可能会增加对过氧化的敏感性。与预期相反，用二十碳五烯酸或二十二碳六烯酸对内皮细胞进行长期修饰不会增加磷脂对过氧化的敏感性。
