Papierz W, Alwasiak J, Kolasa P, Wegrzyn Z, Zakrzewski K, Polis L, Debiec-Rychter M, Liberski P P
Department of Pathological Anatomy, Medical Academy Lodz, Poland.
Ultrastruct Pathol. 1995 May-Jun;19(3):147-66. doi: 10.3109/01913129509064217.
We report here ultrastructural and immunohistochemical studies of neuroblastic differentiation in the retrospective (n = 17) and prospective (n = 26) series of primitive neuroectodermal tumors (PNETs). By electron microscopy, neuritelike structures containing parallel-oriented microtubules, adhesive plaque junctions, and pleomorphic dense-core vesicles were found in the majority of tumor specimens while synaptic specializations were very rare. By immunohistochemistry, synaptophysin appeared to be the most reliable marker for neuroblastic differentiation present in the most reliable marker for neuroblastic differentiation present in the majority of tumors, while 200 kDa neurofilament protein was immunovisualized in a lower proportion of tumors. Glial fibrillary acidic protein (GFAP) was expressed in both reactive astrocytes and in a small proportion of otherwise typical neoplastic cells. We conclude that the majority of PNETs revealed diverse differentiation and that electron microscopy is still the most reliable tool for its detection followed by immunohistochemistry for synaptophysin.
我们在此报告对回顾性（n = 17）和前瞻性（n = 26）系列原始神经外胚层肿瘤（PNETs）进行神经母细胞分化的超微结构和免疫组织化学研究。通过电子显微镜检查，在大多数肿瘤标本中发现了含有平行排列微管、黏附斑连接和多形性致密核心囊泡的神经突样结构，而突触特化则非常罕见。通过免疫组织化学，突触素似乎是大多数肿瘤中存在的神经母细胞分化最可靠的标志物，而200 kDa神经丝蛋白在较低比例的肿瘤中可免疫可视化。胶质纤维酸性蛋白（GFAP）在反应性星形胶质细胞以及一小部分其他方面典型的肿瘤细胞中表达。我们得出结论，大多数PNETs显示出不同的分化，并且电子显微镜仍然是检测其分化的最可靠工具，其次是突触素的免疫组织化学检测。
