[Cellular histamine and bradykinin receptors in the larynx and trachea. I].

Classic physiological and pharmacological experiments were made on 33 rabbits under hexenal anesthesia to determine the presence, topical concentration and activity of H2 and B2-receptors. These parameters were inferred by edema and other vascular responses of the mucosa to local application of histamine and bradykinin prior to and after usage of specific blocker of each receptor type. Both mediators and receptor blockers were applied to laryngeal and tracheal mucosa for 10 min (histamine 10(-4) and 10(-3) g/ml, H2-receptor blocker metiamide 0.1 and 1 mmol, bradykinin 10(-5) and 10(-4) g/ml, B2-receptor blocker pyridinolcarbamate 10(-2) and 10(-1) g/ml). The larynx and trachea were found to contain H2- and B2-receptors the concentrations of which were greater in the subvocal part of the larynx than in the vestibular part and trachea. B2-receptor activity and concentrations in the subvocal part of the larynx were 10 times while in the trachea 1.5-2 times greater than those of H2-receptors. These regularities were not influenced by individual varieties of receptor topical concentrations. The blocker density in the receptors and the duration of their binding were not universally proportional. This is also true for the relations mediator-receptor. Concentrations and activity of H2- and B2-receptors are thought important in pathogenesis and location of acute inflammatory and allergic conditions in the upper respiratory tract.