Slingerland R J, Van Gennip A H, Bodlaender J M, Voûte P A, Van Kuilenburg A B
University of Amsterdam, Department of Pediatrics, The Netherlands.
Biochem Pharmacol. 1995 Jul 17;50(2):277-9. doi: 10.1016/0006-2952(95)00024-t.
Human neuroblastoma SK-N-BE(2)-C cell-line cells were cultured in the presence of various concentrations of cyclopentenyl cytosine (CPEC). In the absence of cytidine, the IC50 value of CPEC for SK-N-BE(2)-C cells was 100 nM after 72 hr drug exposure. The IC20 value was 1 microM after 24 hr of exposure to CPEC in the presence of 10 microM cytidine, whereas in the absence of cytidine, CPEC at 1 microM resulted in an IC40 value after 24 hr. Therefore, cytidine partially prevented the cytostatic effect of CPEC. Cells cultured with 1 microM CPEC for 72 hr were enriched by approximately 410% with mono- and oligonucleosomes in comparison with cells cultured without CPEC. This enrichment was partially prevented with 10 microM deoxycytidine and completely prevented with 10 microM cytidine.
人神经母细胞瘤SK-N-BE(2)-C细胞系细胞在不同浓度的环戊烯基胞嘧啶（CPEC）存在下进行培养。在无胞苷的情况下，药物暴露72小时后，CPEC对SK-N-BE(2)-C细胞的IC50值为100 nM。在存在10 μM胞苷的情况下，暴露于CPEC 24小时后，IC20值为1 μM，而在无胞苷的情况下，1 μM的CPEC在24小时后产生IC40值。因此，胞苷部分地阻止了CPEC的细胞生长抑制作用。与未用CPEC培养的细胞相比，用1 μM CPEC培养72小时的细胞中单核小体和寡核小体富集了约410%。10 μM脱氧胞苷可部分阻止这种富集，10 μM胞苷则可完全阻止。
