Arcari P, Raimo G, Ianniciello G, Gallo M, Bocchini V
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Italy.
Biochim Biophys Acta. 1995 Jul 25;1263(1):86-8. doi: 10.1016/0167-4781(95)00094-w.
An archaeal elongation factor 1 beta gene has been isolated for the first time from a Sulfolobus solfataricus genomic library. The sequenced clone (869 bp) contained two open reading frames, one coding for a protein made of 91 amino acid residues (SsEF-1 beta), the other one encoding a nonidentified product (ORF 115). The amino acid sequences of segments at the N- and C-terminal of the translated SsEF-1 beta were identical to those determined for the native protein. Northern and Southern analyses showed that the SsEF-1 beta gene is represented in S. solfataricus by a unique sequence. Compared to eubacterial or eukaryal corresponding genes the SsEF-1 beta is much shorter.
