Durgam V R, Easton J A, Surya R, Tekmal R R
Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio 78284-7836, USA.
Biochim Biophys Acta. 1995 Jul 25;1263(1):89-92. doi: 10.1016/0167-4781(95)00093-v.
Previous studies have reported the cloning and identification of the int-5, a novel mouse mammary tumor virus (MMTV) integration locus involved in mammary tumorigenesis. Here we report the characterization of the 5.5 kb region from this novel MMTV integration site. Our results show that the region after the MMTV integration site, a 258 bp sequence is homologous (100%) to the mouse early transposon (mETn) long-terminal repeat and other sequences of the this transposon are not present in the 5.5 kb region. Our results also show for the first time the tumor specific expression of mETn and expression of the region downstream of the MMTV integration site that is homologous to mETn-LTR in D2 mammary tumors.
先前的研究报道了int-5的克隆与鉴定，int-5是一个参与乳腺肿瘤发生的新型小鼠乳腺肿瘤病毒（MMTV）整合位点。在此，我们报告了来自这个新型MMTV整合位点的5.5 kb区域的特征。我们的结果表明，在MMTV整合位点之后的区域，一段258 bp的序列与小鼠早期转座子（mETn）的长末端重复序列同源（100%），并且该转座子的其他序列在这个5.5 kb区域中不存在。我们的结果还首次显示了mETn在肿瘤中的特异性表达以及在D2乳腺肿瘤中与mETn-LTR同源的MMTV整合位点下游区域的表达。
