van der Knaap E, Kende H
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824-1312, USA.
Plant Mol Biol. 1995 Jun;28(3):589-92. doi: 10.1007/BF00020405.
Differential display of mRNA was employed to identify gibberellin (GA)-regulated genes in deepwater rice. One of the first differentially displayed products identified was shown to be ten-fold induced after start of GA treatment. The sequence of the clone shows complete amino acid identity with histone H3, and its increased mRNA level correlates with the onset of DNA synthesis. We also identified a gene whose expression pattern did not change over the course of treatment with GA and can be used as standard to correct for loading differences on northern blots.
采用mRNA差异显示技术来鉴定深水水稻中赤霉素（GA）调控的基因。最早鉴定出的差异显示产物之一在GA处理开始后被诱导了10倍。该克隆的序列显示与组蛋白H3具有完全的氨基酸同一性，其mRNA水平的增加与DNA合成的开始相关。我们还鉴定出一个基因，其表达模式在GA处理过程中没有变化，可作为校正Northern印迹上上样差异的标准。
