Mason D Y, Cordell J L, Brown M H, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H
University Department of Cellular Science, John Radcliffe Hospital, Oxford, UK.
Blood. 1995 Aug 15;86(4):1453-9.
The CD79 molecule, comprising two polypeptide chains, mb-1 (CD79a) and B29 (CD79b), is physically associated in the B-cell membrane with immunoglobulin. It transmits a signal after antigen binding and may, therefore, be considered the B cell equivalent of CD3. It appears before the pre-B-cell stage, and the mb-1 (CD79a) chain can still be present at the plasma cell stage. In this report, we describe a new anti-CD79a monoclonal antibody, JCB117, which reacts with human B cells in paraffin embedded tissue sections, including decalcified bone marrow trephines. When tested on a total of 454 paraffin embedded tissue biopsies, gathered from a number of different institutions, it reacted with the great majority (97%) of B-cell neoplasms, covering the full range of B-cell maturation, including 10 of 20 cases of myeloma/plasmacytoma. It is of interest that the antibody labels precursor B-cell acute lymphoblastic leukemia samples, making it the most reliable B-cell marker detectable in paraffin-embedded specimens in this disorder. All neoplasms of T cell or nonlymphoid origin were negative, indicating that antibody JCB117 may be of value to diagnostic histopathologists for the identification of B-cell neoplasms of all maturation stages.
CD79分子由两条多肽链mb-1（CD79a）和B29（CD79b）组成，在B细胞膜上与免疫球蛋白物理相连。抗原结合后它会传递信号，因此可以被视为B细胞中相当于CD3的分子。它在pre-B细胞阶段之前出现，mb-1（CD79a）链在浆细胞阶段仍可能存在。在本报告中，我们描述了一种新的抗CD79a单克隆抗体JCB117，它能与石蜡包埋组织切片中的人B细胞发生反应，包括脱钙骨髓活检组织。在对从多个不同机构收集的总共454份石蜡包埋组织活检样本进行检测时，它与绝大多数（97%）的B细胞肿瘤发生反应，涵盖了B细胞成熟的整个范围，包括20例骨髓瘤/浆细胞瘤中的10例。有趣的是，该抗体标记前体B细胞急性淋巴细胞白血病样本，使其成为在这种疾病的石蜡包埋标本中可检测到的最可靠的B细胞标志物。所有T细胞或非淋巴源性肿瘤均为阴性，这表明抗体JCB117可能对诊断组织病理学家识别所有成熟阶段的B细胞肿瘤有价值。
