Gombart A F, Morosetti R, Miller C W, Said J W, Koeffler H P
Department of Medicine, University of California at Los Angeles (UCLA) School of Medicine, Cedars-Sinai Medical Center 90048, USA.
Blood. 1995 Aug 15;86(4):1534-9.
The tumor suppressor genes p16INK4A and p15INK4B map to the 9p21 chromosomal locus and are either homozygously deleted or mutated in a wide range of human cancer cell lines and tumors. Although chromosome 9 abnormalities have been described in non-Hodgkin's lymphomas (NHLs), to date, the mutational status of these genes has not been determined for these malignancies. A total of five cell lines and 75 NHLs were examined for homozygous deletions or point mutations in the coding regions of both the p15 and p16 genes using Southern blot and/or polymerase chain reaction-single-strand conformation polymorphism analyses. Homozygous deletions of either the p16 gene or both the p15 and p16 genes were observed in one diffuse large B-cell lymphoma cell line and two uncultured lymphomas consisting of one large B-cell and one mixed T-cell lymphoma. In contrast, point mutations were not detected in either the cell lines or lymphomas. These results indicate that the rate of alterations in the p15 and p16 genes is low for lymphomas, but loss of p16 and/or p15 may be involved in the development of some lymphomas.
肿瘤抑制基因p16INK4A和p15INK4B定位于9p21染色体位点，在多种人类癌细胞系和肿瘤中发生纯合缺失或突变。尽管在非霍奇金淋巴瘤（NHL）中已描述了9号染色体异常，但迄今为止，尚未确定这些基因在这些恶性肿瘤中的突变状态。使用Southern印迹法和/或聚合酶链反应-单链构象多态性分析，对总共5个细胞系和75例NHL进行了p15和p16基因编码区纯合缺失或点突变检测。在1个弥漫性大B细胞淋巴瘤细胞系和2例未经培养的淋巴瘤（1例大B细胞淋巴瘤和1例混合性T细胞淋巴瘤）中观察到p16基因或p15和p16基因均存在纯合缺失。相比之下，在细胞系或淋巴瘤中均未检测到点突变。这些结果表明，淋巴瘤中p15和p16基因的改变率较低，但p16和/或p15的缺失可能参与了某些淋巴瘤的发生发展。
