Sutcliffe M J, Jaseja M, Hyde E I, Lu X, Williams J A
Biological NMR Centre, University of Leicester, U.K.
Nat Struct Biol. 1994 Nov;1(11):802-7. doi: 10.1038/nsb1194-802.
Dendroaspin is a short chain neurotoxin homologue from the venom of Elapidae snakes, which lacks neurotoxicity. Unlike neurotoxins, it contains an Arg-Gly-Asp-(RGD)-motif and functions as an inhibitor of platelet aggregation and platelet adhesion with comparable potency to the disintegrins from the venoms of Viperidae. We have determined the structure of dendroaspin in solution using NMR spectroscopy. The structure contains a core similar to that of short chain neurotoxins, but with a novel arrangement of loops and a solvent-exposed RGD-motif. Dendroaspin is thus an integrin antagonist with a well defined fold different from that of the disintegrins, based on the neurotoxin scaffold.
树眼镜蛇毒素是一种来自眼镜蛇科蛇毒的短链神经毒素同系物，它缺乏神经毒性。与神经毒素不同，它含有一个精氨酸 - 甘氨酸 - 天冬氨酸 - （RGD）基序，具有抑制血小板聚集和血小板黏附的功能，其效力与蝰蛇科蛇毒中的去整合素相当。我们利用核磁共振光谱法测定了溶液中树眼镜蛇毒素的结构。该结构包含一个与短链神经毒素相似的核心，但具有新的环排列和一个暴露于溶剂中的RGD基序。因此，基于神经毒素支架，树眼镜蛇毒素是一种整合素拮抗剂，其折叠结构明确，与去整合素不同。
