Van Noorden C J, Jonges G N
Academic Medical Centre, University of Amsterdam, Laboratory of Cell Biology and Histology, The Netherlands.
Histochem Cell Biol. 1995 Feb;103(2):93-101. doi: 10.1007/BF01454005.
In the present review, metabolic compartmentation in liver lobules is discussed as being dynamic and more complex than thus far assumed on the basis of numbers of mRNA or protein molecules or the capacity (zero-order activity) of enzymes. Isoenzyme distribution patterns and local kinetic parameters of enzymes may vary over the different zones of liver lobules. As a consequence, metabolic fluxes in vivo at physiological substrate concentrations may be completely different from those that are assumed on the basis of the number of molecules or the capacity of enzymes present in zones of liver lobules. For a more correct estimation of the levels of metabolic processes in the different compartments of liver tissue, local kinetic parameters and substrate concentrations have to be determined to calculate local metabolic fluxes. Direct measurements of metabolic fluxes in vivo with the use of noninvasive techniques is a promising alternative and the techniques will become increasingly important in future metabolic research.
在本综述中，肝小叶中的代谢区室化被认为是动态的，且比基于mRNA或蛋白质分子数量或酶的容量（零级活性）迄今所假设的更为复杂。同工酶分布模式和酶的局部动力学参数可能在肝小叶的不同区域有所变化。因此，在生理底物浓度下体内的代谢通量可能与基于肝小叶区域中存在的分子数量或酶的容量所假设的通量完全不同。为了更准确地估计肝组织不同区室中的代谢过程水平，必须确定局部动力学参数和底物浓度以计算局部代谢通量。使用非侵入性技术在体内直接测量代谢通量是一种有前景的替代方法，并且这些技术在未来的代谢研究中将变得越来越重要。
