Martin-Morris L E, Henikoff S
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Genetics. 1995 May;140(1):193-9. doi: 10.1093/genetics/140.1.193.
The mechanism underlying trans-inactivation associated with dominant position effect variegation (PEV) of the Drosophila melanogaster brown gene has been addressed by a comparison with its D. virilis homologue. This comparison revealed: 86% identity between conceptual translation products of the brown gene from these two species, functional homology, as the D. virilis gene rescues a D. melanogaster null brown mutation, and conservation of the sequences required for trans-inactivation, as the D. virilis gene in D. melanogaster is subject to dominant PEV. An extended region of sequence similarity upstream of the open reading frame is observed. As the D. virilis homologue is functionally interchangeable with the D. melanogaster gene, these genes must share regulatory sequences as well as protein coding homology. These results support a model in which trans-inactivation is mediated by a heterochromatin-sensitive transcription factor.
通过将果蝇棕色基因与其 virilis 果蝇同源基因进行比较，研究了与果蝇棕色基因显性位置效应斑驳（PEV）相关的反式失活的潜在机制。这种比较揭示了：这两个物种棕色基因的概念翻译产物之间有 86%的同一性、功能同源性，因为 virilis 果蝇基因拯救了果蝇的无棕色突变，以及反式失活所需序列的保守性，因为在果蝇中 virilis 果蝇基因会受到显性 PEV 的影响。在开放阅读框上游观察到一个扩展的序列相似区域。由于 virilis 果蝇同源基因与果蝇基因在功能上是可互换的，这些基因必须共享调控序列以及蛋白质编码同源性。这些结果支持了一个模型，其中反式失活是由异染色质敏感转录因子介导的。
