Kalinin V L, Kuznetsova L V
Genetika. 1995 Jun;31(6):784-7.
The lethal and mutagenic effects of hydrogen peroxide H2O (2-20 mM), cumene hydroperoxide (0.2-2.0 mM), and potassium permanganate KMnO4 (0.25-1.0 mM) on the heat-inducible lambda cI857 prophage were studied under conditions of heat-induction immediately after the mutagenic treatment of lysogenic cells of Escherichia coli oxyR+ or oxyR delta 3. Within the range of the doses used, these agents decreased prophage survival by 3-5 orders of magnitude and increased mutation frequency by up to 0.2% under the action of hydrogen peroxide and cumene hydroperoxide, and up to 1.5% in the case of KMnO4 (in oxyR+ cells). In the absence of the inducible OxyR system of oxidative stress, both lethal and mutagenic effects of H2O were enhanced. The oxyR delta 3 mutation increased lethal and mutagenic effects of cumene hydroperoxide and KMnO4 only at the highest concentrations used. Apparently, the OxyR system does not repair lesions induced by oxidative agents, but only prevents their formation.
研究了过氧化氢H₂O（2 - 20 mM）、氢过氧化异丙苯（0.2 - 2.0 mM）和高锰酸钾KMnO₄（0.25 - 1.0 mM）对热诱导型λcI857原噬菌体的致死和诱变作用，实验条件为在诱变处理大肠杆菌oxyR⁺或oxyRΔ3的溶原细胞后立即进行热诱导。在所使用的剂量范围内，这些试剂使原噬菌体存活率降低3 - 5个数量级，并在过氧化氢和氢过氧化异丙苯作用下使突变频率增加高达0.2%，在KMnO₄作用下（在oxyR⁺细胞中）增加高达1.5%。在缺乏可诱导的氧化应激OxyR系统的情况下，H₂O的致死和诱变作用均增强。oxyRΔ3突变仅在所用最高浓度下增加了氢过氧化异丙苯和KMnO₄的致死和诱变作用。显然，OxyR系统不能修复由氧化试剂诱导的损伤，而只是防止其形成。
