Hyslop P A, Hinshaw D B, Scraufstatter I U, Cochrane C G, Kunz S, Vosbeck K
Department of Central Nervous System Research, Eli Lilly & Co., Indianapolis, IN 46285, USA.
Free Radic Biol Med. 1995 Jul;19(1):31-7. doi: 10.1016/0891-5849(95)00005-i.
Host defense against bacterial pathogens in higher organisms is mediated in part by the generation of reactive oxygen species (ROS) by PMN. In this study, we determined the following effects of exposure of constant concentrations of H2O2 on E. coli in a culture continuously monitored for H2O2 concentration, numbers, and viabilities of cells: (1) E. coli growth rates monitored for 1 h were profoundly affected by concentrations of H2O2, between 25-50 microM. (2) Complete bacteriostasis was observed at 100 microM. (3) Significant cell killing was not observed until the concentration of H2O2 was greater than 500 microM. (4) Bacteriostatic (25-50 microM) concentrations of H2O2 appeared not to be toxic to human skin fibroblasts for a 2-h exposure. (4) Bacteriostasis by H2O2 could not be explained by metabolic inhibition, because intracellular ATP levels were not compromised at bacteriostatic doses of H2O2. (5) Measurements of H2O2 concentrations in subcutaneous abscess fluid infected with both E. coli and S. aureus indicated prevailing concentrations of the oxidant consistent with a proposed role of H2O2 in host defense.
高等生物对细菌病原体的宿主防御部分是由中性粒细胞产生的活性氧（ROS）介导的。在本研究中，我们测定了在持续监测H2O2浓度、细胞数量和活力的培养物中，恒定浓度的H2O2对大肠杆菌的以下影响：（1）在25-50微摩尔之间，监测1小时的大肠杆菌生长速率受到H2O2浓度的深刻影响。（2）在100微摩尔时观察到完全抑菌。（3）直到H2O2浓度大于500微摩尔时才观察到显著的细胞杀伤。（4）对于2小时的暴露，抑菌浓度（25-50微摩尔）的H2O2似乎对人皮肤成纤维细胞无毒。（4）H2O2的抑菌作用不能用代谢抑制来解释，因为在抑菌剂量的H2O2下细胞内ATP水平没有受到损害。（5）对感染大肠杆菌和金黄色葡萄球菌的皮下脓肿液中H2O2浓度的测量表明，氧化剂的普遍浓度与H2O2在宿主防御中的作用一致。
