尿α1-球蛋白组分在尿路结石形成中的作用。特别提及血清α1-球蛋白对碳酸钙悬浮液的聚集作用。
Role of urinary alpha1-globulin fraction in formation of urinary calculi. With special reference to aggregating effect of serum alpha1-globulin on calcium carbonate suspension.
作者信息
Kimura Y, Kisaki N
出版信息
Urol Int. 1977;32(6):428-36. doi: 10.1159/000280159.
The present study was undertaken to determine if urinary protein can produce urinary stones and what types of protein have such an effect. Normal human serum was fractionated first with ammonium sulfate and then with DEAE-cellulose. The stone-forming action of each fraction was estimated by calculating their ability to alter the sedimentation rate and zeta-potential of a calcium carbonate suspension. Finally, the proteins in each fraction were identified by cellulose acetate electrophoresis. The most marked aggregating and precipitating effect on a CaCO3 suspension was observed with a mixture of proteins of alpha1-globulin and albumin fractions. However, beta- and gamma-fractions had little aggregating effect on the CaCO3 suspension.
本研究旨在确定尿蛋白是否会产生尿路结石以及何种类型的蛋白质具有这种作用。首先用硫酸铵对正常人血清进行分级分离，然后用DEAE - 纤维素进行分级分离。通过计算各组分改变碳酸钙悬浮液沉降速率和zeta电位的能力来评估其结石形成作用。最后，通过醋酸纤维素电泳鉴定各组分中的蛋白质。观察到α1 - 球蛋白和白蛋白组分的蛋白质混合物对碳酸钙悬浮液具有最显著的聚集和沉淀作用。然而，β - 和γ - 组分对碳酸钙悬浮液几乎没有聚集作用。
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