Buttini M, Limonta S, Luyten M, Boddeke H
Sandoz Pharma Ltd., Basel, Switzerland.
Histochem J. 1995 Apr;27(4):291-9. doi: 10.1007/BF00398971.
The distribution of the mRNAs encoding the different isoforms of the catalytic subunit (A subunit) of calcineurin has been investigated in rat thymus and kidney using in situ hybridization histochemistry with specific antisense oligonucleotide probes. In the thymus, the mRNAs of the A beta isoforms were the predominant transcripts and showed very intense hybridization signals in the cortical areas. The A alpha mRNAs were expressed at low levels. A beta 2 mRNA was expressed at higher levels than A beta 3 mRNA, but no difference could be detected between the expression levels of A alpha 1 and A alpha 2. In the kidney, highest calcineurin A mRNA hybridization signals were found in the medulla. Signal intensities of A alpha mRNAs were comparable to those of A beta mRNAs. A alpha 1 mRNA level was extremely weak, and A beta 2 mRNA expression was slightly higher than A beta 3 mRNA expression. A tissue-specific distribution pattern of the alternatively spliced isoforms of calcineurin A, as suggested by these preliminary data from thymus and kidney, may be critical in understanding the toxic side-effects associated with the use of the immunosuppressive, calcineurin-inhibiting compounds cyclosporin A and FK506.
利用特异性反义寡核苷酸探针原位杂交组织化学技术，研究了大鼠胸腺和肾脏中编码钙调神经磷酸酶催化亚基（A亚基）不同同工型的mRNA的分布情况。在胸腺中，Aβ同工型的mRNA是主要转录本，在皮质区域显示出非常强烈的杂交信号。Aα mRNA表达水平较低。Aβ2 mRNA的表达水平高于Aβ3 mRNA，但Aα1和Aα2的表达水平之间未检测到差异。在肾脏中，在髓质中发现最高的钙调神经磷酸酶A mRNA杂交信号。Aα mRNA的信号强度与Aβ mRNA相当。Aα1 mRNA水平极其微弱，Aβ2 mRNA的表达略高于Aβ3 mRNA的表达。胸腺和肾脏的这些初步数据表明，钙调神经磷酸酶A可变剪接同工型的组织特异性分布模式，对于理解与使用免疫抑制性钙调神经磷酸酶抑制化合物环孢素A和FK506相关的毒副作用可能至关重要。
