Arenas M I, Fraile B, De Miguel M, Paniagua R
Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain.
Histochem J. 1995 Apr;27(4):329-37. doi: 10.1007/BF00398976.
A light and electron microscope immunocytochemical study and Western blotting analysis has been performed on intermediate filaments (vimentin, desmin and cytokeratins) in the testis of the teleost fish Gambusia affinis holbrooki. An immunoreaction to vimentin was observed in the epithelium of the efferent ducts, testicular canal and their surrounding peritubular cells. Positive vimentin immunostaining was also observed in the cells located around seminiferous tubules (boundary cells), Leydig cells, interstitial fibroblasts, chromatophores, and blood vessel endothelial cells. In contrast to mammals, no vimentin immunoreactivity was found in the Sertoli cells. Immunoreactivity to desmin was weak in the epithelial cells of the efferent ducts and testicular canal and intense in the peritubular cells that surrounded these ducts. Desmin immunoreactivity was also observed in the seminiferous tubule boundary cells. The immunoreactivity was weak in the boundary cells that surrounded germ cell cysts containing spermatogonia or spermatocytes and intense in the boundary cells around cysts with elongated or mature spermatids. Immunoreactivity towards cytokeratins was observed only in testicular blood vessels. Cytokeratin immunolabelling was intense in the endothelium and weak in the vascular smooth muscle cells. No cytokeratin immunoreactivity was found in the Sertoli cells, germ cells, interstitial cells or in the efferent duct epithelium. The absence of intermediate filaments in the Sertoli cells, the absence of cytokeratins in the epithelium of the sperm excretory ducts, and the presence of desmin filaments in these epithelial cells are the most important differences with regards to the intermediate filament phenotype in mammalian testes.
对食蚊鱼（Gambusia affinis holbrooki）睾丸中的中间丝（波形蛋白、结蛋白和细胞角蛋白）进行了光镜和电镜免疫细胞化学研究以及蛋白质免疫印迹分析。在输出小管、睾丸管上皮及其周围的管周细胞中观察到波形蛋白免疫反应。在生精小管周围的细胞（边界细胞）、睾丸间质细胞、间质成纤维细胞、色素细胞和血管内皮细胞中也观察到波形蛋白阳性免疫染色。与哺乳动物不同，在支持细胞中未发现波形蛋白免疫反应性。结蛋白在输出小管和睾丸管上皮细胞中的免疫反应性较弱，而在围绕这些管道的管周细胞中较强。在生精小管边界细胞中也观察到结蛋白免疫反应性。在围绕含有精原细胞或精母细胞的生殖细胞囊肿的边界细胞中，免疫反应性较弱，而在含有伸长或成熟精子细胞的囊肿周围的边界细胞中较强。仅在睾丸血管中观察到细胞角蛋白免疫反应性。细胞角蛋白免疫标记在内皮细胞中较强，在血管平滑肌细胞中较弱。在支持细胞、生殖细胞、间质细胞或输出小管上皮中未发现细胞角蛋白免疫反应性。支持细胞中缺乏中间丝、精子排泄管上皮中缺乏细胞角蛋白以及这些上皮细胞中存在结蛋白丝，是与哺乳动物睾丸中间丝表型相比最重要的差异。
