Borghese R, Wall J D
Biochemistry Department, University of Missouri-Columbia 65211, USA.
J Bacteriol. 1995 Aug;177(15):4549-52. doi: 10.1128/jb.177.15.4549-4552.1995.
In a recent report identifying the promoters of the Rhodobacter capsulatus glnBA operon, it was suggested that an internal promoter upstream of the glnA gene probably resulted in different levels of glnBA and glnA transcripts (D. Foster-Hartnett and R. G. Kranz, J. Bacteriol. 176:5171-5176, 1994). Therefore, to investigate the regulation, we constructed and examined the expression of a number of translational fusions in R. capsulatus glnBA. The results support a role for posttranscriptional regulation.
在最近一份鉴定荚膜红细菌谷氨酰胺合成酶基因操纵子(glnBA operon)启动子的报告中,有人提出glnA基因上游的一个内部启动子可能导致了glnBA和glnA转录本水平的差异(D. 福斯特 - 哈特内特和R. G. 克兰兹,《细菌学杂志》176:5171 - 5176,1994年)。因此,为了研究其调控机制,我们构建并检测了荚膜红细菌glnBA中多个翻译融合体的表达情况。结果支持了转录后调控的作用。