利用Tn5tac1从菊花欧文氏菌EC16突变体中克隆一个编码高碱性、富含天冬酰胺的果胶酸裂解酶同工酶的pel基因，该突变体存在影响主要果胶酸裂解酶同工酶的缺失。
Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes.
作者信息
Alfano J R, Ham J H, Collmer A
机构信息
Department of Plant Pathology, Cornell University, Ithaca, New York 14853-4203, USA.
出版信息
J Bacteriol. 1995 Aug;177(15):4553-6. doi: 10.1128/jb.177.15.4553-4556.1995.
Abstract
Erwinia chrysanthemi mutant CUCPB5047, delta(pelA pelE) delta(pelB pelC)::28bp delta(pelX) delta 4bp pehX::omega Cmr, was constructed, mutated with Tn5tac1, and screened for isopropyl-beta-D-thiogalactopyranoside-dependent pectate lyase (Pel) production. A Kmr SacI fragment from the hyperexpressing Pel+ mutant CUCPB5066 was cloned into Escherichia coli and sequenced. The gene identified, pelL, encodes a novel, asparagine-rich, highly alkaline enzyme that is similar in primary structure to PelX and in enzymological properties to PelE.
摘要
构建了菊欧文氏菌突变体CUCPB5047，即Δ(pelA pelE) Δ(pelB pelC)::28bp Δ(pelX) Δ4bp pehX::ωCmr，用Tn5tac1进行诱变，并筛选对异丙基-β-D-硫代半乳糖苷依赖的果胶酸裂解酶（Pel）的产生情况。从超表达Pel+突变体CUCPB5066中克隆出一个Kmr SacI片段并导入大肠杆菌中进行测序。鉴定出的基因pelL编码一种新型的、富含天冬酰胺的高碱性酶，其一级结构与PelX相似，酶学性质与PelE相似。
相似文献
1
Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes.利用Tn5tac1从菊花欧文氏菌EC16突变体中克隆一个编码高碱性、富含天冬酰胺的果胶酸裂解酶同工酶的pel基因，该突变体存在影响主要果胶酸裂解酶同工酶的缺失。
J Bacteriol. 1995 Aug;177(15):4553-6. doi: 10.1128/jb.177.15.4553-4556.1995.
2
Characterization of the exopolygalacturonate lyase PelX of Erwinia chrysanthemi 3937.菊欧文氏菌3937的胞外多聚半乳糖醛酸裂解酶PelX的特性分析
J Bacteriol. 1999 Mar;181(5):1652-63. doi: 10.1128/JB.181.5.1652-1663.1999.
3
Characterization of the pelL gene encoding a novel pectate lyase of Erwinia chrysanthemi 3937.编码菊欧文氏菌3937新型果胶酸裂解酶的pelL基因的特性分析
Mol Microbiol. 1995 Jun;16(6):1183-95. doi: 10.1111/j.1365-2958.1995.tb02341.x.
4
Genetic analysis of the pelA-pelE cluster encoding the acidic and basic pectate lyases in Erwinia chrysanthemi EC16.对菊欧文氏菌EC16中编码酸性和碱性果胶酸裂解酶的pelA-pelE基因簇的遗传分析。
Mol Gen Genet. 1987 Oct;209(3):615-7. doi: 10.1007/BF00331172.
5
Regulation of pelZ, a gene of the pelB-pelC cluster encoding a new pectate lyase of Erwinia chrysanthemi 3937.pelZ基因的调控，pelZ基因属于pelB - pelC基因簇，编码菊欧文氏菌3937的一种新型果胶酸裂解酶。
J Bacteriol. 1996 Dec;178(24):7187-96. doi: 10.1128/jb.178.24.7187-7196.1996.
6
Comparative analysis of the five major Erwinia chrysanthemi pectate lyases: enzyme characteristics and potential inhibitors.五种主要菊欧文氏菌果胶裂解酶的比较分析：酶特性及潜在抑制剂
J Bacteriol. 1997 Apr;179(8):2503-11. doi: 10.1128/jb.179.8.2503-2511.1997.
7
Erwinia chrysanthemi EC16 Produces a Second Set of Plant-Inducible Pectate Lyase Isozymes.菊欧文氏菌 EC16 产生第二组植物诱导性果胶裂解酶同工酶。
Appl Environ Microbiol. 1993 Jun;59(6):1756-61. doi: 10.1128/aem.59.6.1756-1761.1993.
8
The exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937.外多聚半乳糖醛酸裂解酶PelW和寡聚半乳糖醛酸裂解酶Ogl，是菊欧文氏菌3937中果胶分解代谢的两种细胞质酶。
J Bacteriol. 1999 Jul;181(13):3912-9. doi: 10.1128/JB.181.13.3912-3919.1999.
9
Effect of mutations in the T1.5 loop of pectate lyase A from Erwinia chrysanthemi EC16.来自菊欧文氏菌EC16的果胶酸裂解酶A的T1.5环突变的影响
Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1339-42. doi: 10.1107/s0907444903011491. Epub 2003 Jun 27.
10
Analysis of Erwinia chrysanthemi EC16 pelE::uidA, pelL::uidA, and hrpN::uidA mutants reveals strain-specific atypical regulation of the Hrp type III secretion system.对菊欧文氏菌EC16的pelE::uidA、pelL::uidA和hrpN::uidA突变体的分析揭示了Hrp III型分泌系统的菌株特异性非典型调控。
Mol Plant Microbe Interact. 2004 Feb;17(2):184-94. doi: 10.1094/MPMI.2004.17.2.184.
引用本文的文献
1
Biochemical Characterization of a Pectate Lyase AnPL9 from Aspergillus nidulans.从构巢曲霉中分离的果胶裂解酶 AnPL9 的生化特性分析。
Appl Biochem Biotechnol. 2022 Dec;194(12):5627-5643. doi: 10.1007/s12010-022-04036-x. Epub 2022 Jul 8.
2
PaeX, a second pectin acetylesterase of Erwinia chrysanthemi 3937.PaeX，菊欧文氏菌3937的第二种果胶乙酰酯酶。
J Bacteriol. 2003 May;185(10):3091-100. doi: 10.1128/JB.185.10.3091-3100.2003.
3
Bacterial Pathogens in Plants: Life up against the Wall.植物中的细菌病原体：逆境求生
Plant Cell. 1996 Oct;8(10):1683-1698. doi: 10.1105/tpc.8.10.1683.
4
Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome.果胶酸裂解酶A，一种嗜纤维梭菌纤维小体的酶亚基。
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4125-9. doi: 10.1073/pnas.071045598. Epub 2001 Mar 20.
5
The exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937.外多聚半乳糖醛酸裂解酶PelW和寡聚半乳糖醛酸裂解酶Ogl，是菊欧文氏菌3937中果胶分解代谢的两种细胞质酶。
J Bacteriol. 1999 Jul;181(13):3912-9. doi: 10.1128/JB.181.13.3912-3919.1999.
6
Characterization of the exopolygalacturonate lyase PelX of Erwinia chrysanthemi 3937.菊欧文氏菌3937的胞外多聚半乳糖醛酸裂解酶PelX的特性分析
J Bacteriol. 1999 Mar;181(5):1652-63. doi: 10.1128/JB.181.5.1652-1663.1999.
7
HrpW of Erwinia amylovora, a new harpin that contains a domain homologous to pectate lyases of a distinct class.梨火疫欧文氏菌的HrpW，一种新的类过敏蛋白，含有一个与特定类别的果胶酸裂解酶同源的结构域。
J Bacteriol. 1998 Oct;180(19):5203-10. doi: 10.1128/JB.180.19.5203-5210.1998.
8
Pectate lyase PelI of Erwinia chrysanthemi 3937 belongs to a new family.菊欧文氏菌3937的果胶酸裂解酶PelI属于一个新家族。
J Bacteriol. 1997 Dec;179(23):7321-30. doi: 10.1128/jb.179.23.7321-7330.1997.
9
Cloning, molecular characterization and expression pattern of a strawberry ripening-specific cDNA with sequence homology to pectate lyase from higher plants.一个与高等植物果胶裂解酶具有序列同源性的草莓成熟特异性cDNA的克隆、分子特征及表达模式
Plant Mol Biol. 1997 Aug;34(6):867-77. doi: 10.1023/a:1005847326319.
10
Regulation of pelZ, a gene of the pelB-pelC cluster encoding a new pectate lyase of Erwinia chrysanthemi 3937.pelZ基因的调控，pelZ基因属于pelB - pelC基因簇，编码菊欧文氏菌3937的一种新型果胶酸裂解酶。
J Bacteriol. 1996 Dec;178(24):7187-96. doi: 10.1128/jb.178.24.7187-7196.1996.
本文引用的文献
1
Formation of the blue pigment indigoidine by phytopathogenic erwinia.植物致病性欧文氏菌产生蓝色色素靛蓝素。
Appl Microbiol. 1966 Nov;14(6):870-2. doi: 10.1128/am.14.6.870-872.1966.
2
Erwinia chrysanthemi EC16 Produces a Second Set of Plant-Inducible Pectate Lyase Isozymes.菊欧文氏菌 EC16 产生第二组植物诱导性果胶裂解酶同工酶。
Appl Environ Microbiol. 1993 Jun;59(6):1756-61. doi: 10.1128/aem.59.6.1756-1761.1993.
3
Multiplication and Virulence in Plant Tissues of Escherichia coli Clones Producing Pectate Lyase Isozymes PLb and PLe at High Levels and of an Erwinia chrysanthemi Mutant Deficient in PLe.高水平产生果胶裂解酶同工酶 PLb 和 PLe 的大肠杆菌克隆和一株缺失 PLe 的欧文氏菌突变体在植物组织中的增殖和毒力。
Appl Environ Microbiol. 1987 Oct;53(10):2315-20. doi: 10.1128/aem.53.10.2315-2320.1987.
4
Activity stain for rapid characterization of pectic enzymes in isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gels.等电聚焦和十二烷基硫酸钠-聚丙烯酰胺凝胶中果胶酶的快速特性分析的活性染色。
Appl Environ Microbiol. 1985 Sep;50(3):615-22. doi: 10.1128/aem.50.3.615-622.1985.
5
The Three-Dimensional Structure of Pectate Lyase E, a Plant Virulence Factor from Erwinia chrysanthemi.来自菊欧文氏菌的植物致病因子果胶酸裂解酶E的三维结构
Plant Physiol. 1994 Nov;106(3):849-862. doi: 10.1104/pp.106.3.849.
6
Resolution of four pectate lyase structural genes of Erwinia chrysanthemi (EC16) and characterization of the enzymes produced in Escherichia coli.菊欧文氏菌（EC16）四个果胶酸裂解酶结构基因的解析及在大肠杆菌中产生的酶的特性分析。
Mol Gen Genet. 1987 Sep;209(2):319-25. doi: 10.1007/BF00329660.
7
Specific interactions of Erwinia chrysanthemi KdgR repressor with different operators of genes involved in pectinolysis.菊欧文氏菌KdgR阻遏物与参与果胶分解的不同基因操纵子的特异性相互作用。
J Mol Biol. 1994 Feb 18;236(2):427-40. doi: 10.1006/jmbi.1994.1155.
8
Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71.胡萝卜软腐欧文氏菌胡萝卜软腐亚种71的一个新型果胶酸裂解酶基因（pel-3）和一个紧密连锁的内切多聚半乳糖醛酸酶基因（peh-1）的核苷酸序列及表达
Appl Environ Microbiol. 1994 Jul;60(7):2545-52. doi: 10.1128/aem.60.7.2545-2552.1994.
9
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
10
Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter.可通过插入的外源启动子去阻遏作用进行扩增的低拷贝数质粒克隆载体。
Gene. 1984 Apr;28(1):45-54. doi: 10.1016/0378-1119(84)90086-6.
Zotero 插件