用于测定烟草花叶病毒（一种杆状病毒）中多胺的改进方法。

Improved method for polyamine determination in TMV, a rod-shaped virus.

作者信息

Torrigiani P, Rabiti A L, Betti L, Marani F, Brizzi M, Bagni N, Canova A

机构信息

Dipartimento di Biologia evoluzionistica sperimentale, University of Bologna, Italy.

出版信息

J Virol Methods. 1995 May;53(1):157-63. doi: 10.1016/0166-0934(95)00008-i.

DOI:10.1016/0166-0934(95)00008-i

PMID:7635924

Abstract

Polyamines were measured in viruses using different techniques. An improved method of polyamine analysis is reported for tobacco mosaic virus (TMV), a rod-shaped virus (95% protein and 5% RNA), based on HPLC of sonicated PCA-treated highly purified virus suspensions. This method allowed higher and more reliable recovery of TMV polyamines (putrescine, spermidine and spermine) when compared to the HPLC of non-sonicated samples and to thin layer chromatography. It is suggested that sonication acts on PCA-precipitated protein aggregates causing the release of trapped polyamine molecules.

摘要

使用不同技术对病毒中的多胺进行了测量。本文报道了一种改进的多胺分析方法，用于烟草花叶病毒（TMV），这是一种杆状病毒（95%为蛋白质，5%为RNA），该方法基于对经PCA处理的超声破碎的高度纯化病毒悬液进行高效液相色谱分析。与未超声处理的样品的高效液相色谱分析和薄层色谱法相比，该方法能实现更高且更可靠的TMV多胺（腐胺、亚精胺和精胺）回收率。研究表明，超声处理作用于PCA沉淀的蛋白质聚集体，导致被困的多胺分子释放出来。