Strauss W S, Gschwend M H, Sailer R, Schneckenburger H, Steiner R, Rück A
Institut für Lasertechnologien in der Medizin an der Universität Ulm, Germany.
J Photochem Photobiol B. 1995 May;28(2):155-61. doi: 10.1016/1011-1344(94)07082-y.
Meso-tetra(4-sulphonatophenyl)porphyrin (TPPS4) taken up by cells is mainly localized in lysosomes as previously shown by fluorescence microscopical and fluorescence spectroscopical investigations. In the present study the intracellular fluorescence behaviour and the intracellular amount of this dye at various growth periods of cells were examined. For cells irradiated in the growth phase a relocalization of TPPS4 from the lysosomes into the cytoplasm and finally into the nucleus was observed. In contrast, for cells irradiated in the stationary phase no redistribution could be detected and therefore no evidence for severe damage of the lysosomal membranes and subsequently for the release of lytical enzymes is given. In both cases lethal damage of the cells was achieved as examined using the trypan blue exclusion test. This indicates that damage of the lysosomes is less important in the photodynamic inactivation of cells sensitized by TPPS4.
如先前荧光显微镜和荧光光谱研究所示，细胞摄取的中-四(4-磺基苯基)卟啉(TPPS4)主要定位于溶酶体中。在本研究中，检测了该染料在细胞不同生长时期的细胞内荧光行为和细胞内含量。对于在生长阶段照射的细胞，观察到TPPS4从溶酶体重新定位到细胞质，最终进入细胞核。相反，对于在静止期照射的细胞，未检测到重新分布，因此没有证据表明溶酶体膜受到严重损伤，进而没有证据表明有溶酶体酶的释放。在这两种情况下，使用台盼蓝排斥试验检测到细胞发生了致死性损伤。这表明在TPPS4致敏的细胞光动力失活中，溶酶体损伤不太重要。
