Auclair D, Sowerbutts S F, Setchell B P
Department of Animal Sciences, Waite Agricultural Research Institute, University of Adelaide, South Australia.
J Reprod Fertil. 1995 May;104(1):17-26. doi: 10.1530/jrf.0.1040017.
The long-term effects of active immunization against testosterone were studied in rams, with particular reference to blood concentrations of gonadotrophin and testosterone, spermatogenesis, testis blood flow and mating behaviour. Ten 18-month-old Merino rams, kept on pasture, were studied for 1 year. Every 2 months, five rams received injections of BSA in Freund's adjuvant and five other rams were treated with testosterone-3(o-carboxymethyl)oxime-BSA as immunogen. Anti-testosterone antibodies (mean titre: 1:4484 +/- 582, after boosters) were maintained in the circulation, with the help of regular booster injections. In time, immunization reduced live mass in testosterone-immunized rams; however, there was no effect on testicular volume throughout the whole study. In testosterone-immunized rams, significantly higher concentrations of gonadotrophins were found in jugular venous plasma, as well as increased concentrations of total plasma testosterone. LH pulse frequency, amplitude and nadir were increased significantly in testosterone-immunized rams. After 12 months of immunization, no differences were found in the number of spermatozoa per ejaculate, in daily sperm production or in testis mass between the two groups of rams; however, testicular blood flow (per testis) and epididymis mass were significantly reduced in testosterone-immunized rams. Testosterone immuno-neutralization also resulted in a significant reduction in the number of mounts culminating in ejaculation performed during a 10 min trial carried out on a number of occasions during the experiment. Additional information on these rams was collected 3 months after castration. However, there were no significant differences in mean plasma LH and FSH concentrations, either before, or after, a single GnRH injection between the two groups of rams at this time.
在公羊中研究了针对睾酮主动免疫的长期影响，特别涉及促性腺激素和睾酮的血浓度、精子发生、睾丸血流及交配行为。对10只18月龄、饲养在牧场的美利奴公羊进行了为期1年的研究。每2个月，5只公羊接受弗氏佐剂中牛血清白蛋白（BSA）注射，另外5只公羊用睾酮-3（邻羧甲基）肟-BSA作为免疫原进行处理。借助定期加强注射，抗睾酮抗体（加强注射后平均效价：1:4484±582）维持在循环中。随着时间推移，免疫使睾酮免疫公羊的体重减轻；然而，在整个研究过程中对睾丸体积没有影响。在睾酮免疫公羊中，颈静脉血浆中促性腺激素浓度显著升高，同时血浆总睾酮浓度也升高。睾酮免疫公羊的促黄体生成素（LH）脉冲频率、幅度和最低点显著增加。免疫12个月后，两组公羊每次射精的精子数量、每日精子生成量或睾丸质量均未发现差异；然而睾酮免疫公羊的睾丸血流（每侧睾丸）和附睾质量显著降低。睾酮免疫中和还导致在实验期间多次进行的10分钟试验中，以射精告终的爬跨次数显著减少。在去势3个月后收集了这些公羊的更多信息。然而，此时两组公羊在单次促性腺激素释放激素（GnRH）注射之前或之后，平均血浆LH和促卵泡生成素（FSH）浓度均无显著差异。
