乙型肝炎病毒核心启动子的体内活性：组织特异性和时间调控。
In vivo activity of the hepatitis B virus core promoter: tissue specificity and temporal regulation.
作者信息
Billet O, Grimber G, Levrero M, Seye K A, Briand P, Joulin V
机构信息
Institut National de la Santé et de la Recherche Médicale U-380, Institut Cochin de Génétique Moléculaire, Paris, France.
出版信息
J Virol. 1995 Sep;69(9):5912-6. doi: 10.1128/JVI.69.9.5912-5916.1995.
Abstract
The contribution of the hepatitis B virus enhancers I and II in the regulation of the activity of the core and the X promoters was assessed in transgenic mice. Surprisingly, despite the presence of heterologous promoters linked 5' of the X gene, the transgene expression is mostly due to core promoter (Cp) activity present in the X coding sequence. Moreover, the restriction of Cp activity to hepatic tissue required the combined action of both enhancers I and II, whereas the proximity of these two enhancers was insufficient to confer tissue specificity on Xp activity. Furthermore, the liver-specific activity of the Cp was developmentally regulated in an enhancer I-independent manner.
摘要
在转基因小鼠中评估了乙型肝炎病毒增强子I和II对核心启动子和X启动子活性调节的贡献。令人惊讶的是，尽管在X基因的5'端连接了异源启动子，但转基因表达主要归因于X编码序列中存在的核心启动子（Cp）活性。此外，将Cp活性限制在肝组织需要增强子I和II的共同作用，而这两个增强子的接近度不足以赋予Xp活性组织特异性。此外，Cp的肝脏特异性活性以不依赖增强子I的方式受到发育调控。
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本文引用的文献
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