睾丸精原细胞瘤：通过流式细胞术研究核脱氧核糖核酸倍体模式的临床意义。

Testicular seminoma: clinical significance of nuclear deoxyribonucleic acid ploidy pattern as studied by flow cytometry.

作者信息

Mor Y, Leibovich I, Raviv G, Nass D, Medalia O, Goldwasser B, Nativ O

机构信息

Department of Urology, Chaim Sheba Medical Center, Tel Hashomer, Israel.

出版信息

J Urol. 1995 Sep;154(3):1041-3; discussion 1044. doi: 10.1016/s0022-5347(01)66970-2.

DOI:10.1016/s0022-5347(01)66970-2

PMID:7637050

Abstract

PURPOSE

We evaluated the clinical significance of deoxyribonucleic acid (DNA) ploidy pattern as a predictor of prognosis in patients with testicular seminoma.

MATERIALS AND METHODS

Flow cytometric nuclear DNA analysis was performed on archival specimens from 65 patients with pure seminoma who underwent radical orchiectomy between 1970 and 1992.

RESULTS

A total of 42 specimens (65%) exhibited a DNA diploid pattern, while 23 (35%) were DNA aneuploid. Diploidy was manifested in 73% of the stage I tumors versus 31% of stage II cancers (p = 0.004). No correlation was found between ploidy and histological type, size or local extension of the tumor. Tumor progression was observed in 5 patients, exclusively displaying aneuploid histograms (p = 0.0017), and 3 of them subsequently died of the disease.

CONCLUSIONS

DNA ploidy pattern may provide important prognostic information for patients with testicular seminoma.

摘要

目的

我们评估了脱氧核糖核酸（DNA）倍体模式作为睾丸精原细胞瘤患者预后预测指标的临床意义。

材料与方法

对1970年至1992年间接受根治性睾丸切除术的65例纯精原细胞瘤患者的存档标本进行流式细胞术核DNA分析。

结果

共有42份标本（65%）呈现DNA二倍体模式，而23份（35%）为DNA非整倍体。二倍体在73%的I期肿瘤中出现，而在II期癌症中为31%（p = 0.004）。未发现倍体与肿瘤的组织学类型、大小或局部扩展之间存在相关性。5例患者出现肿瘤进展，均表现为非整倍体直方图（p = 0.0017），其中3例随后死于该疾病。