蛋白激酶Cδ基因在生殖细胞中的表达。
Expression of protein kinase C delta gene in germ cells.
作者信息
Um J Y, Choi B M, Kim J S, Rim J S, Kim H M, Chung H T
机构信息
Department of Microbiology and Immunology, Wonkwang University School of Medicine, Iksan, Korea.
出版信息
J Urol. 1995 Sep;154(3):1237-40.
PURPOSE
To investigate the biological functions of protein kinase C delta (PKC delta) in spermatogenesis.
MATERIALS AND METHODS
We examined PKC delta transcript in mouse testis by means of in situ hybridization and Northern blotting.
RESULTS
In testes of normal mice, signals of PKC delta gene expression were detected specifically at the spermatid stage. The PKC delta gene was weakly expressed in 8-week-old mice and highly expressed by 12 weeks. However, the expression was not detected in testes of germ cell-deficient W/Wv mice even at 12 weeks.
CONCLUSIONS
Protein kinase C delta gene expression may be controlled by specific developmental processes and PKC delta may play a role in spermatogenesis.
目的
研究蛋白激酶Cδ（PKCδ）在精子发生中的生物学功能。
材料与方法
我们通过原位杂交和Northern印迹法检测了小鼠睾丸中的PKCδ转录本。
结果
在正常小鼠睾丸中，PKCδ基因表达信号在精子细胞阶段特异性检测到。PKCδ基因在8周龄小鼠中弱表达，在12周龄时高表达。然而，即使在12周龄时，在生殖细胞缺陷的W/Wv小鼠睾丸中也未检测到该表达。
结论
蛋白激酶Cδ基因表达可能受特定发育过程控制，且PKCδ可能在精子发生中起作用。
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