Nakatani K, Kitahata L M, Harada Y, Omote K, Collins J G, Yuge O
Department of Anesthesiology, Hiroshima Prefectural Hiroshima Hospital.
Masui. 1995 Jun;44(6):795-9.
The present study examined interactions between mu and delta opiate subtypes and serotonin. Noxious activity evoked by radiant heat (51 degrees C, 8 sec) was extracellularly recorded from single discriminated wide dynamic range (WDR) neurons in decerebrate spinally transected cats. DAGO (mu selective opioid agonist) 1 microgram or DPDPE (delta selective opioid agonist) 30 micrograms was combined with serotonin (n = 6 each) 250 micrograms. The dose of each drug by itself when administered intrathecally produced no suppression of noxiously evoked activity. Although the combination of DAGO and serotonin produced no significant suppression of noxiously evoked activity, DPDPE and serotonin produced significant suppression to 72.8 +/- 8.0 % (mean +/- SEM) of control values (P < 0.01). Intravenously administered naloxone 0.1 mg reversed the suppression produced by the DPDPE-serotonin combination. Our results suggest that combinations of serotonin and delta selective opiates may be more effective in suppressing noxiously evoked activity than combinations with mu selective opiates.
本研究检测了μ和δ阿片亚型与5-羟色胺之间的相互作用。在去大脑脊髓横断猫中，从单个鉴别出的广动力范围（WDR）神经元细胞外记录由辐射热（51摄氏度，8秒）诱发的伤害性活动。将1微克的DAGO（μ选择性阿片样激动剂）或30微克的DPDPE（δ选择性阿片样激动剂）与250微克的5-羟色胺联合使用（每组n = 6）。鞘内单独给药时，每种药物的剂量均未产生对伤害性诱发活动的抑制作用。虽然DAGO和5-羟色胺的联合使用未产生对伤害性诱发活动的显著抑制作用，但DPDPE和5-羟色胺联合使用可使伤害性诱发活动显著抑制至对照值的72.8±8.0%（平均值±标准误）（P<0.01）。静脉注射0.1毫克纳洛酮可逆转DPDPE-5-羟色胺联合用药所产生的抑制作用。我们的结果表明，5-羟色胺与δ选择性阿片类药物联合使用在抑制伤害性诱发活动方面可能比与μ选择性阿片类药物联合使用更有效。
