妊娠相关胎盘蛋白4在人胎盘、脐带及成年女性生殖组织中的免疫组化定位。

Immunohistochemical localization of pregnancy-related placental protein 4 in human placenta, umbilical cord and adult human female genital tissues.

作者信息

Gocze P M, Jozsa R, Szabó D G, Bohn H, Freeman D A

机构信息

University Medical School, Pecs, Hungary.

出版信息

Placenta. 1995 Apr;16(3):309-16. doi: 10.1016/0143-4004(95)90117-5.

DOI:10.1016/0143-4004(95)90117-5

PMID:7638111

Abstract

Placental protein 4 (PP4) is a soluble placental tissue protein which was isolated from human placenta. The aim of the present study was to demonstrate the localization of cells containing PP4 in human placenta and in various female genital tissues under normal conditions. PP4 immunoreactive structures were demonstrated by using the peroxidase-antiperoxidase immunohistochemical technique. The samples were obtained from normal human placenta, umbilical cord, uterine cervix, endometrium, ovary and vulva. The most differentiated trophoblastic cells, the syncytiotrophoblasts, as well as the intermediate trophoblast cells contained PP4. PP4 immunoreactivity was present in umbilical cord as well. Occasionally PP4 was detected in normal ovarian, endometrial or vulvar tissue samples. Cervix and myometrium were free of PP4 immunoreactive material. PP4 staining was cytoplasmic. Our findings indicate that PP4 cannot be considered specific for the placenta since it is present in some human adult tissues as well.

摘要

胎盘蛋白4（PP4）是一种从人胎盘中分离出的可溶性胎盘组织蛋白。本研究的目的是在正常条件下，证明含PP4的细胞在人胎盘和各种女性生殖组织中的定位。采用过氧化物酶-抗过氧化物酶免疫组织化学技术显示PP4免疫反应性结构。样本取自正常人体胎盘、脐带、子宫颈、子宫内膜、卵巢和外阴。分化程度最高的滋养层细胞，即合体滋养层细胞以及中间滋养层细胞含有PP4。脐带中也存在PP4免疫反应性。偶尔在正常卵巢、子宫内膜或外阴组织样本中检测到PP4。子宫颈和子宫肌层没有PP4免疫反应性物质。PP4染色位于细胞质中。我们的研究结果表明，PP4不能被认为是胎盘特有的，因为它也存在于一些成人组织中。