Gołab E
Zakład Parazytologii Lekarskiej, PZH, Warszawa.
Wiad Parazytol. 1995;41(1):13-8.
The polymerase chain reaction (PCR) is used for in vitro amplification of specific DNA fragments. The PCR technique is based on the reiteration of a three-step process: denaturation DNA into single strands, annealing primers (specific synthetic oligonucleotides) to the DNA template and enzymatic extension from the primers along the templates. The usefulness of this new technique to the detection of Toxoplasma gondii DNA has been described.
聚合酶链反应（PCR）用于体外扩增特定的DNA片段。PCR技术基于一个三步过程的重复：将DNA变性为单链，使引物（特定的合成寡核苷酸）与DNA模板退火，并沿着模板从引物进行酶促延伸。已经描述了这项新技术在检测弓形虫DNA方面的用途。
