Wygnanski T, Desatnik H, Quigley H A, Glovinsky Y
Goldschleger Eye Institute, Sheba Medical Center, Tel Hashomer, Israel.
Am J Ophthalmol. 1995 Aug;120(2):184-9. doi: 10.1016/s0002-9394(14)72606-6.
To compare the loss of ganglion cells to cone loss in the macular area of monkey eyes with experimental glaucoma.
Experimental glaucoma was induced in eyes of three cynomolgus monkeys by argon laser applications to the trabecular meshwork. The average duration +/- S.D. of glaucoma in the experimental animals was 3.8 +/- 0.5 months. Glaucomatous damage was estimated, before the animals were killed, by evaluating stereoscopic photographs of the optic disk and red-free photographs of the nerve fiber layer. Ganglion cell nucleoli and cone pedicles were counted by using light microscopy from slides containing strips of retinal tissue. Cell density was then calculated by using the modified Abercrombie formula. Percent loss of ganglion cells and cones was determined from three eyes of three monkeys in 300-micron segments that were 900 to 1,200 microns superior and inferior to the foveal center.
Cone loss was not found at 4.5 to 6 degrees eccentricity above and below the fovea, whether damage was mild or severe. The average loss +/- S.D. of ganglion cells was 81.1% +/- 10.7%, whereas the average cone loss was only 3.5% +/- 6.4%.
Marked parafoveal cone loss was not found in experimental glaucoma in which extensive damage to ganglion cells occurred.
比较实验性青光眼猴眼黄斑区神经节细胞丢失与视锥细胞丢失情况。
对三只食蟹猴的眼睛通过氩激光照射小梁网诱导实验性青光眼。实验动物青光眼的平均持续时间±标准差为3.8±0.5个月。在处死动物前，通过评估视盘的立体照片和神经纤维层的无赤光照片来估计青光眼性损害。使用光学显微镜对含有视网膜组织条带的载玻片计数神经节细胞核仁和视锥细胞蒂。然后使用改良的阿伯克龙比公式计算细胞密度。从三只猴子的三只眼睛中，在距中央凹中心上下900至1200微米、300微米节段中确定神经节细胞和视锥细胞的丢失百分比。
在中央凹上方和下方4.5至6度偏心度处，无论损害是轻度还是重度，均未发现视锥细胞丢失。神经节细胞的平均丢失±标准差为81.1%±10.7%，而视锥细胞的平均丢失仅为3.5%±6.4%。
在发生广泛神经节细胞损害的实验性青光眼中，未发现明显的中央凹旁视锥细胞丢失。
