Grover J, Roughley P J
Genetics Unit, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada.
Biochem J. 1995 Aug 1;309 ( Pt 3)(Pt 3):963-8. doi: 10.1042/bj3090963.
The expression of six cell-surface proteoglycans (syndecan, fibroglycan, amphiglycan, glypican, betaglycan and CD44) was studied at the mRNA level. Analysis was performed by Northern blotting using total RNA preparations from freshly isolated articular chondrocytes obtained from both juveniles and adults. Similar results were obtained for both age groups. By far the most abundant message was that for amphiglycan, CD44 message was next in relative abundance, and the messages for fibroglycan, glypican and betaglycan were all expressed at low levels. Syndecan message could not be detected by this technique. This pattern of expression was different to that observed in cultured skin fibroblasts, where the messages for amphiglycan, CD44, fibroglycan and glypican were all expressed at a similar level. In contrast with the fibroblasts, where the amphiglycan message exhibits no size polymorphism, the chondrocyte amphiglycan message is present in three polymorphic forms, due to the use of alternative polyadenylation signals. When the newly isolated chondrocytes are maintained in monolayer culture for several passages, the amphiglycan message heterogeneity reverts to that characteristic of the fibroblasts. Thus human articular chondrocytes are characterized by both their high level of amphiglycan message expression and their use of alternative polyadenylation signals.
在mRNA水平上研究了六种细胞表面蛋白聚糖（多功能蛋白聚糖、纤维蛋白聚糖、双栖蛋白聚糖、磷脂酰肌醇蛋白聚糖、β-蛋白聚糖和CD44）的表达。使用从幼年和成年个体新鲜分离的关节软骨细胞制备的总RNA，通过Northern印迹法进行分析。两个年龄组得到了相似的结果。到目前为止，表达量最丰富的是双栖蛋白聚糖的信息，CD44的信息相对丰度次之，而纤维蛋白聚糖、磷脂酰肌醇蛋白聚糖和β-蛋白聚糖的信息表达水平都很低。用这种技术检测不到多功能蛋白聚糖的信息。这种表达模式与在培养的皮肤成纤维细胞中观察到的不同，在皮肤成纤维细胞中，双栖蛋白聚糖、CD44、纤维蛋白聚糖和磷脂酰肌醇蛋白聚糖的信息表达水平相似。与成纤维细胞不同，成纤维细胞中双栖蛋白聚糖的信息没有大小多态性，由于使用了不同的聚腺苷酸化信号，软骨细胞的双栖蛋白聚糖信息以三种多态形式存在。当新分离的软骨细胞在单层培养中传代培养几代后，双栖蛋白聚糖信息的异质性恢复为成纤维细胞的特征性异质性。因此，人关节软骨细胞的特征在于其双栖蛋白聚糖信息的高表达水平以及对不同聚腺苷酸化信号的使用。
