Schmitt J, Mielke R, Schrewe H
Max-Planck-Institute for Immunobiology, Department of Developmental Biology, Freiburg, Germany.
Biochem Biophys Res Commun. 1995 Aug 4;213(1):211-7. doi: 10.1006/bbrc.1995.2118.
We have characterized the genomic organization of a mouse type I activin receptor. Using the mouse tsk7L cDNA, 4 overlapping lambda clones containing the activin receptor IA (ActRIA) gene were isolated from a mouse 129 Sv genomic library. The mouse ActRIA gene is encoded by 10 exons and spans approximately 40 kb. The size of the introns was determined and the intron/exon boundaries were sequenced. Primer extension analysis of the 5' non-translated region using RNA from different organs or tissues revealed a strong transcription start site 68 nucleotides upstream of the ATG. Knowledge of the structure of the ActRIA gene is essential for the production of ActRIA deficient mice by homologous recombination.
我们已经对小鼠I型激活素受体的基因组结构进行了表征。利用小鼠tsk7L cDNA，从129 Sv小鼠基因组文库中分离出4个包含激活素受体IA（ActRIA）基因的重叠λ克隆。小鼠ActRIA基因由10个外显子编码，跨度约为40 kb。确定了内含子的大小并对内含子/外显子边界进行了测序。使用来自不同器官或组织的RNA对5'非翻译区进行引物延伸分析，发现在ATG上游68个核苷酸处有一个强转录起始位点。ActRIA基因结构的知识对于通过同源重组产生ActRIA缺陷小鼠至关重要。
