Mori S, Claesson-Welsh L, Okuyama Y, Saito Y
Department of Laboratory Medicine, Yamagata University School of Medicine, Japan.
Biochem Biophys Res Commun. 1995 Aug 4;213(1):32-9. doi: 10.1006/bbrc.1995.2094.
The platelet-derived growth factor beta-receptor undergoes polyubiquitination as a consequence of ligand binding. In the present study, we have examined the ligand-induced receptor ubiquitination also in the other receptor tyrosine kinase (structurally different) subfamilies by immunoblotting with anti-ubiquitin antiserum. In addition to the platelet-derived growth factor alpha- and beta-receptors, all the monomeric receptor tyrosine kinases examined, such as the receptors for epidermal growth factor (subfamily I), colony stimulating factor-1 (subfamily III), and fibroblast growth factor (subfamily IV), were found to be ubiquitinated after ligand stimulation. However, the insulin receptor (subfamily II), which is a tetrameric molecule, was not. These data suggest that the ligand-induced polyubiquitination of the receptor is a general phenomenon observed in most of the monomeric receptor tyrosine kinases.
血小板衍生生长因子β受体因配体结合而发生多聚泛素化。在本研究中，我们还通过用抗泛素抗血清进行免疫印迹，在其他受体酪氨酸激酶（结构不同）亚家族中检测了配体诱导的受体泛素化。除了血小板衍生生长因子α受体和β受体外，所有检测的单体受体酪氨酸激酶，如表皮生长因子受体（亚家族I）、集落刺激因子-1受体（亚家族III）和成纤维细胞生长因子受体（亚家族IV），在配体刺激后均被发现发生了泛素化。然而，作为四聚体分子的胰岛素受体（亚家族II）却没有。这些数据表明，配体诱导的受体多聚泛素化是在大多数单体受体酪氨酸激酶中观察到的普遍现象。
