Tilson M D
Department of Surgery, St. Luke's/Roosevelt Hospital Center, Columbia University, New York, NY 10583, USA.
Biochem Biophys Res Commun. 1995 Aug 4;213(1):40-3. doi: 10.1006/bbrc.1995.2095.
Immunoglobulin G (IgG) from human aneurysmal aorta was used to partially purify an aortic protein with an apparent MW approximately 80 kDa. Amino acid sequencing of a tryptic digest revealed two sequences with homology to mouse tenascin-X. The autoimmune IgG was then shown to react with purified human tenascin, and a rabbit polyclonal anti-human tenascin antibody was found to react with the purified autoantigen. These observations suggest that the autoantigen of abdominal aortic aneurysm disease may be homologous to a calcium-binding member of the tenascin superfamily that has been identified by others in pig and cow.
来自人动脉瘤主动脉的免疫球蛋白G（IgG）被用于部分纯化一种表观分子量约为80 kDa的主动脉蛋白。对胰蛋白酶消化产物进行氨基酸测序，发现了两个与小鼠腱生蛋白-X具有同源性的序列。随后发现自身免疫性IgG与纯化的人腱生蛋白发生反应，并且发现兔抗人腱生蛋白多克隆抗体与纯化的自身抗原发生反应。这些观察结果表明，腹主动脉瘤疾病的自身抗原可能与腱生蛋白超家族中一种钙结合成员同源，其他人已在猪和牛中鉴定出该成员。
