Ishida T, Bounds S V, Caldwell J
Department of Pharmacology and Toxicology, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, England.
Chirality. 1995;7(4):278-84. doi: 10.1002/chir.530070415.
Racemic trans-anethole epoxide [1-(4'-methoxyphenyl)-propane-1,2-oxide] was incubated with water, buffers, and rat liver microsomes and cytosol and the stereochemistry of the diols produced was determined by HPLC as their dicamphanyl esters. The diol metabolites were isolated by HPLC from the urine of rats administered [1'-14C] trans-anethole and their stereochemistry determined after derivatization to their camphanyl esters. The stereochemical course of the metabolism of trans-anethole by rat liver microsomes and cytosol is discussed.
外消旋反式茴香脑环氧化物[1-(4'-甲氧基苯基)-丙烷-1,2-氧化物]与水、缓冲液以及大鼠肝微粒体和胞液一起孵育，所产生二醇的立体化学通过高效液相色谱法作为其双樟脑基酯来测定。二醇代谢物通过高效液相色谱法从给予[1'-14C]反式茴香脑的大鼠尿液中分离出来，并在衍生化为其樟脑基酯后确定其立体化学。讨论了大鼠肝微粒体和胞液对外消旋反式茴香脑代谢的立体化学过程。
