Alibardi L
Department of Histology and Embryology, University of Sydney, NSW, Australia.
Bull Assoc Anat (Nancy). 1995 Mar;79(244):3-9.
In order to study the pattern of growth of regenerating cartilage, H3-thymidine was injected in individuals of the lizards Anolis carolinensis and Lampropholis delicata during advanced stages of tail regeneration. Light and electron microscopic autoradiographical analysis showed that the regenerating cartilaginous tube was mostly derived from the interstitial proliferation of chondroblasts. Appositional growth is limited during the initial stages of cartilage formation. Later, after the differentiation of an inner and outer perichondrium, a few cells were added to the cartilaginous tube while cellular degeneration of the calcifying areas was in progress. In this way a constant diameter of the cartilaginous tube was maintained. In order to study the rate of production of matrix protein (collagen), H3-proline was administered to other samples of Lampropholis. To the best of the author's knowledge this was done for the first time in the cartilage of a reptiles. The tracier was mostly localized in the apical-intermediate cartilage and one hour postinjection it was mostly concentrated in the ergastoplasm around the Golgi apparatus and its secretory vesicles. Three hours after the injection of H3-proline some of the tracier was found in the cytoplasm of chondrocytes and in the extracellular matrix.
为了研究再生软骨的生长模式，在尾再生的晚期阶段，向绿安乐蜥和精美柔蜥个体注射³H-胸腺嘧啶核苷。光镜和电镜放射自显影分析表明，再生软骨管主要源自软骨母细胞的间质增殖。在软骨形成的初始阶段，表面增生有限。后来，在内外软骨膜分化后，当钙化区域发生细胞变性时，有一些细胞添加到软骨管中。通过这种方式，软骨管的直径得以维持。为了研究基质蛋白（胶原蛋白）的产生速率，向精美柔蜥的其他样本注射³H-脯氨酸。据作者所知，这是首次在爬行动物的软骨中进行此项操作。示踪剂大多定位于顶端-中间软骨，注射后一小时，它大多集中在高尔基体及其分泌小泡周围的内质网中。注射³H-脯氨酸三小时后，在软骨细胞的细胞质和细胞外基质中发现了一些示踪剂。
