血小板衍生生长因子-BB和胰岛素样生长因子-I对培养的大鼠胸主动脉外膜成纤维细胞中纤连蛋白表达的调控

Regulation of fibronectin expression by PDGF-BB and IGF-I in cultured rat thoracic aortic adventitial fibroblasts.

作者信息

Zhang J, Lo C

机构信息

Department of Physiology, Uniformed Services University of the Health Science, Bethesda, MD 20814, USA.

出版信息

Cell Biol Int. 1995 Jun;19(6):517-25. doi: 10.1006/cbir.1995.1096.

DOI:10.1006/cbir.1995.1096

PMID:7640666

Abstract

Regulation of fibronectin (FN) by Platelet-derived Growth Factor-BB (PDGF-BB) and Insulin-like Growth Factor-I (IGF-I) in cultured rat thoracic aortic adventitial fibroblasts were examined. PDGF-BB enhances FN mRNA levels in a time and concentration-dependent fashion. The effect of IGF-I on PDGF-BB-induced FN levels was also examined. There was a larger increase in FN mRNA levels (4-fold) in the cells treated with both IGF-I and PDGF-BB than with either IGF-I (2-fold) or PDGF-BB (2-fold) alone. The effects of PDGF-BB and IGF-I on FN levels were examined. There was a larger increase in FN levels (135%) in the cells treated with both PDGF-BB and IGF-I than with either PDGF-BB (43%) or IGF-I (45%) alone. Regulation of adventitial fibroblast FN may lead to blood vessel diseases and/or angiogenesis.

摘要

研究了血小板衍生生长因子 -BB（PDGF -BB）和胰岛素样生长因子 -I（IGF -I）对培养的大鼠胸主动脉外膜成纤维细胞中纤连蛋白（FN）的调控作用。PDGF -BB以时间和浓度依赖性方式提高FN mRNA水平。还研究了IGF -I对PDGF -BB诱导的FN水平的影响。与单独使用IGF -I（2倍）或PDGF -BB（2倍）相比，同时用IGF -I和PDGF -BB处理的细胞中FN mRNA水平增加幅度更大（4倍）。检测了PDGF -BB和IGF -I对FN水平的影响。与单独使用PDGF -BB（43%）或IGF -I（45%）相比，同时用PDGF -BB和IGF -I处理的细胞中FN水平增加幅度更大（135%）。外膜成纤维细胞FN的调控可能导致血管疾病和/或血管生成。