Ray R B, Steele R, Seftor E, Hendrix M
Department of Internal Medicine, Saint Louis University School of Medicine, Missouri 63110, USA.
Cancer Res. 1995 Sep 1;55(17):3747-51.
We have identified previously a gene from a human cervical carcinoma cell (HeLa) cDNA expression library that encodes a M(r) approximately 37,000 c-myc promoter-binding protein (MBP-1), which binds to the TATA box sequences of c-myc P2 promoter and exerts a negative regulatory function by down-regulating c-myc expression. In normal human tissues, this cloned gene showed variable expression. In this study, we have demonstrated that introduction of the MBP-1 gene into human breast carcinoma cells reduced their ability to invade through a basement membrane matrix in vitro but did not alter their growth rate. Human breast carcinoma transfected with MBP-1 cells showed a loss of anchorage-independent growth and also suppressed tumor formation in athymic nude mice. These results suggest the possible involvement of MBP-1 as a tumor suppressor gene in human breast carcinoma cells.
我们先前已从人宫颈癌细胞（HeLa）cDNA表达文库中鉴定出一个基因，该基因编码一种分子量约为37,000的c-myc启动子结合蛋白（MBP-1），它与c-myc P2启动子的TATA盒序列结合，并通过下调c-myc表达发挥负调控功能。在正常人体组织中，该克隆基因表现出可变表达。在本研究中，我们已证明将MBP-1基因导入人乳腺癌细胞可降低其体外穿透基底膜基质的能力，但不改变其生长速率。转染了MBP-1的人乳腺癌细胞显示出失去锚定非依赖性生长，并且还抑制了无胸腺裸鼠中的肿瘤形成。这些结果表明MBP-1可能作为一种肿瘤抑制基因参与人乳腺癌细胞的过程。
