Sher E, Rosa P, Bassetti M, Zanini A
CNR Center of Cytopharmacology, Department of Medical Pharmacology, University of Milan, Italy.
Eur J Cell Biol. 1995 May;67(1):15-22.
RINm5F, a rat insulin-secreting pancreatic cell line, responds to nerve growth factor (NGF) by extending neurite-like processes. Secretogranin II (SgII), a marker of neuroendocrine secretory organelles, has recently been found to be a good marker of neuronal differentiation in both human neuroblastoma and rat pheochromocytoma cells. The present paper reports the results obtained from immunocytochemical studies, which show that NGF increases the expression of SgII-immunolabeled organelles in RINm5F cells. We also demonstrate that NGF increases the expression of insulin and that SgII and insulin are predominantly, but not always, colocalized. These results suggest that this insulinoma cell line may be a good model for studying the role of SgII in neuroendocrine secretion mechanisms.
RINm5F是一种大鼠胰岛素分泌胰腺细胞系，它通过延伸类神经突样突起对神经生长因子（NGF）作出反应。分泌粒蛋白II（SgII）是神经内分泌分泌细胞器的标志物，最近发现它在人类神经母细胞瘤和大鼠嗜铬细胞瘤细胞中都是神经元分化的良好标志物。本文报道了免疫细胞化学研究的结果，这些结果表明NGF增加了RINm5F细胞中SgII免疫标记细胞器的表达。我们还证明NGF增加了胰岛素的表达，并且SgII和胰岛素主要但并非总是共定位的。这些结果表明，这种胰岛素瘤细胞系可能是研究SgII在神经内分泌分泌机制中作用的良好模型。
